Background: The Ketogenic Diet (KD) promotes metabolic changes and optimizes energy
metabolism. It is unknown if microRNAs (miRs) are influenced by KD in obese subjects. The
screening of circulating miRs was performed with the FDA approved platform n-counter flex and
blood biochemical parameters were dosed by ADVIA 1800.
Objectives: The aim of this study was to evaluate mir profile under 6 weeks of biphasic KD in obese
subjects. We enrolled 36 obese subjects (18 females and 18 males) in stage 1 of Edmonton Obesity
Staging System (EOSS) parameter.
Result: Any correlation was found between biochemical parameter and three miRs, hsa-let-7b-5p,
hsa-miR-143-3p and hsa-miR-504-5p influenced in an equal manner in both sexes. The KD resulted
safe and ameliorate both biochemical and anthropometric factors in obese subjects re-collocating
them into stage 0 of EOSS parameters.
Conclusion: The miRs herein identified under KD might be a useful tool to monitor low carbohydrate
nutritional regimens which reflect indirectly the regulatory biochemical mechanisms and cell
signaling that orchestrate metabolic and signaling pathways.