Background: Trichilia silvatica, popularly known as “catiguá-branco”, is distributed in
Brazil (Mato Grosso do Sul), and members of this genus are commonly used for the treatment of
rheumatism (arthritis). The aim of this research was to investigate the anti-inflammatory, antioxidant
and antiproliferative activities of the methanolic extract of the leaves (MEL-TS) and bark (MEB-TS)
of T. silvatica. We also evaluated the concentration of phenolic compounds, flavonoids, flavonol, and
condensed tannins by liquid chromatography - photodiode array (LC/PDA) analysis.
Methods: The MEL-TS and MEB-TS revealed the presence of caffeic acid in both extracts by
LC/PDA. The samples were evaluated for antioxidant activity using free-radical scavenging and
lipoperoxidation assays. The anti-inflammatory effects were studied in carrageenan-induced paw
edema, pleurisy and zymosan-induced arthritis.
Results: The MEL-TS and MEB-TS showed the total phenolic concentration (270.8 ± 17.10 mg gallic
acid equivalents GAE/g extract and 278.8 ± 25.13 mg GAE/ g extract, respectively), and flavonoids in
MEL-TS (209.30 ± 2.91 mg quercetin equivalents QE/ g extract). In the lipoperoxidation assay, exhibited
moderate antioxidant activity with IC50 values ≤ 35.32 µg/mL. Both extracts inhibited oedema induced
by carrageenan at 2 h and 4 h, inhibited leukocyte migration at 6 h post administration, and did
not impact zymosan-induced arthritis. Finally, MEL-TS was particularly effective against prostate cell
line (GI50 ≤ 0.22 µg/mL).
Conclusion: Overall, the results indicated that T. silvatica reduce migration leukocytes activity, edema
formation in these models of experimental arthritis could explain the popular use for treatment of inflammatory