Anti-inflammatory, Antioxidant and Antiproliferative Activities from Trichilia silvatica (C.DC)

Author(s): Jacenir V. Da Silva, Rafael C. dos Santos, Pedro C.O. Júnior, Milena M.C. Pederiva, Maria do Carmo Vieira, Candida A. L. Kassuya, Claudia A.L. Cardoso, Zefa V. Pereira, Ana L.T.G. Ruiz, Mary Ann Foglio, João E. De Carvalho, Anelise S.N. Formagio*.

Journal Name: Current Pharmaceutical Biotechnology

Volume 19 , Issue 12 , 2018

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Abstract:

Background: Trichilia silvatica, popularly known as “catiguá-branco”, is distributed in Brazil (Mato Grosso do Sul), and members of this genus are commonly used for the treatment of rheumatism (arthritis). The aim of this research was to investigate the anti-inflammatory, antioxidant and antiproliferative activities of the methanolic extract of the leaves (MEL-TS) and bark (MEB-TS) of T. silvatica. We also evaluated the concentration of phenolic compounds, flavonoids, flavonol, and condensed tannins by liquid chromatography - photodiode array (LC/PDA) analysis.

Methods: The MEL-TS and MEB-TS revealed the presence of caffeic acid in both extracts by LC/PDA. The samples were evaluated for antioxidant activity using free-radical scavenging and lipoperoxidation assays. The anti-inflammatory effects were studied in carrageenan-induced paw edema, pleurisy and zymosan-induced arthritis.

Results: The MEL-TS and MEB-TS showed the total phenolic concentration (270.8 ± 17.10 mg gallic acid equivalents GAE/g extract and 278.8 ± 25.13 mg GAE/ g extract, respectively), and flavonoids in MEL-TS (209.30 ± 2.91 mg quercetin equivalents QE/ g extract). In the lipoperoxidation assay, exhibited moderate antioxidant activity with IC50 values ≤ 35.32 µg/mL. Both extracts inhibited oedema induced by carrageenan at 2 h and 4 h, inhibited leukocyte migration at 6 h post administration, and did not impact zymosan-induced arthritis. Finally, MEL-TS was particularly effective against prostate cell line (GI50 ≤ 0.22 µg/mL).

Conclusion: Overall, the results indicated that T. silvatica reduce migration leukocytes activity, edema formation in these models of experimental arthritis could explain the popular use for treatment of inflammatory processes (rheumatism).

Keywords: Catigua-branco, arthritis, lipid peroxidation, phenolic acids, prostate cancer, radical scavenging, oedema.

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Article Details

VOLUME: 19
ISSUE: 12
Year: 2018
Page: [973 - 981]
Pages: 9
DOI: 10.2174/1389201020666181123121817
Price: $58

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