Background: The prevalence of anti-drug resistance by disease causing microorganisms
has necessitated the search for alternative sources of drugs for the treatment of the ailments caused
by these microorganisms. This study examines the biological properties of extracts from the leaves
of Irvingia gabonensis (bush mango).
Objective: The objective of this study is to determine the anti-microbial activity of chloroform
fraction of the leaf extract and compare it with that of clinical reference.
Method: Antimicrobial activity of the chloroform fraction of the leaf extract of Irvingia gabonensis
was evaluated against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Shigella
dysenteriae, Salmonella typhi, Klebsiella pneumonia, Salmonella paratyphi, Candida albicans
and Trichophyton rubrum by using the agar well diffusion method and Mycobacterium tuberculosis
using agar proportion method on Lowenstein–Jensen medium. Preliminary phytochemical
screening of the chloroform leaf fraction was done using qualitative standard methods.
Result: This showed the presence of saponins, flavonoids, tannins, coumarin, phenol and alkaloids.
Organisms were susceptible to chloroform fraction at different concentrations. The lowest MIC
value obtained was 0.625mg/mL for S. aureus and S. typhi. While, five out of seven mycobacterial
strains that were used, were susceptible.
Conclusion: The antimicrobial activity is a result of the phytochemicals present in leaf. Therefore,
we conclude that Irvingia gabonensis leaves can be used in the development of new pharmaceuticals
research activities such as drug production.