Background: Epilepsy is one of the most common and severe brain disorders in the
world, characterized by recurrent spontaneous seizures due to an imbalance between cerebral excitability
and inhibition. Oxidative stress is a biochemical state in which reactive oxygen species are
generated and associated with various diseases including epilepsy. Pycnogenol, a polyphenol obtained
from the pine tree and has antioxidant& anti-inflammatory activity. So, the aim of the study
was to evaluate the effect of Pycnogenol on pentylenetetrazole (PTZ)-induced seizures in mice.
Methods: The mice of swiss strain each weighing 18-30g were used. Pycnogenol (50&100mg/kg)
was suspended in carboxymethyl cellulose in saline and administered orally. Diazepam (1mg/kg,
i.p) was used as a standard drug. The anticonvulsant effects of the drugs were measured using PTZ
and cognitive behaviour was also assessed. The biochemical estimation was done by measuring
Thiobarbituric acid, Superoxide dismutase, Catalase, and reduced glutathione followed by the
Result: Pycnogenol 50 & 100mg/kg showed a significant increase in latency to PTZ-induced seizures,
decrease in duration and frequency of convulsions compared to control animals; however, the
effects were dose-dependent and were more significant at a higher dose. No impairment in cognitive
functions like memory and muscle relaxant was observed following pycnogenol 50 & 100
mg/kg. The effect of Pycnogenol on biochemical parameter was found to be significant. It significantly
(p<0.01) decreases the level of TBARS and increases the levels of SOD, catalase, and GSH
in the brain tissue. The histopathological evaluation showed less neuronal degeneration in the brain
due to PTZ-induced seizures in comparison to control group.
Conclusion: Thus pycnogenol has a protective approach towards convulsion and can be included as
an adjuvant therapy with antiepileptic drugs.