Background: Moringa oleifera kernel oil consist of monounsaturated fatty acid with high
percentage of oleic acid. The oil consist of phytochemicals, bioactive compounds and nutrients that
have several application in health industries. However, the oil degrades on exposure to light, heat and
oxygen overtime. In addition, rancidity cause the oil quality to defect and reduce the shelf-life.
Therefore, microencapsulation techniques are uniquely applied to oil to preserve their native quality
and prolong their shelf life.
Objective: This study examines different polymer concentrations and injection flowrates of zein nanofiber
from Moringa oleifera kernel oil using coaxial electrospinning method.
Methods: A 40% w/v zein polymer was the optimal loading concentration and 0.7 mL/hour of zein
polymer with 0.1 mL/hour of Moringa oleifera kernel oil was the optimal injection flowrates for
electrospun nanofiber. Analysis of the Moringa oleifera kernel oil and polymer sample micromorphology,
were investigated with Field Emission Scanning Electron Microscopy (FESEM) and transmission
electron microscopy (TEM).
Result: result shows uniformly layered nanofiber. The nanofiber has no beads formation and the fiber
strands are continuous with no entanglement. The polymer encapsulated the oil efficiently. Furthermore,
thermal analysis through Differential Scanning Calorimetry (DSC) showed consistency in
the nanofiber thermal behavior. Thermogravimetric (TGA) analysis revealed the weight loss and
thermal dissociation of the polymer structure. The electrospun nanofiber average diameter was 450 ±
24 nm and exhibited hydrophobicity.
Conclusion: The co-axial electrospine technique was effective in fabricating electrospune nanofibers.