It is now well established that protein sumoylation is an important mechanism
to regulate multiple cellular processes including gene transcription, chromatin structure,
cell proliferation and differentiation, as well as pathogenesis. In the vertebrate eye, we
and others have previously shown that sumoylation can regulate differentiation of major
ocular tissues including retina and lens. However, the expression patterns of the three
types of sumoylation enzymes, the activating enzymes SAE1 and UBA2, the conjugating
enzyme UBC9, and the ligating enzymes such as RanBP2 and PIAS1 have not been
well studied in the ocular tissues. In the present study, using QRT-PCR and western blot
analysis, we have determined the differentiatial expression patterns of the above three
types of enzymes, and the obtained results lay down a foundation for further exploration
of sumoylation functions in vertebrate eye.
Keywords: SAE1, UBA2, UBC9, PIAS1, RanBP2, Sumoylation, sumoylation enzymes.
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