Background: Clostridium tetani are rod-like, anaerobic types of pathogenic bacteria of
the genus Clostridium. It is Gram-positive in nature and appears as a tennis racket or drumsticks
on staining with the dye. Tetanus is a neuromuscular disease wherein the Clostridium tetani
exotoxin produces muscle fits in the host. Tetanus is the second leading cause of worldwide deaths
occurring from the family of immunization-preventable diseases.
Methods: In this research, subtractive proteome analysis of C. tetani was performed to identify
putative drug targets. The proteins were subjected to blast analysis against Homo sapiens to
exclude homologous proteins. The database of Essential Genes was used to determine the essential
proteins of the pathogen. These basic proteins were additionally analyzed to anticipate the
corresponding metabolic pathways.
Results: Cellular localization analysis was carried out to determine the possibility of the protein
presence in the outer membrane. The study has recognized 29 essential genes and 20 unique
pathways of 2314 proteins as potential drug targets. There are 29 essential proteins, out of which, 3
membrane proteins were also identified as putative drug targets.
Conclusion: Virtual screening in contrast to these proteins can be valuable in the identification of
novel clinical compounds for the C. tetani infections in Homo sapiens.