Background: The relationship between protein structure and its bioactivity is one of the fundamental
problems for protein engineering and pharmaceutical design.
Method: A new method, called SPTD (Simulated Protein Thermal Detection), was proposed for studying and
improving the thermal stability of enzymes. The method was based on the evidence observed by conducting the
MD (Molecular Dynamics) simulation for all the atoms of an enzyme vibrating from the velocity at a room temperature
(e.g., 25°C) to the desired working temperature (e.g., 65°C). According to the recorded MD trajectories
and the coordinate deviations of the constituent residues under the two different temperatures, some new strategies
have been found that are useful for both drug delivery and starch industry.
Conclusion: The SPTD technique presented in this paper may become a very useful tool for pharmaceutical
design and protein engineering.
Keywords: Simulated protein thermal detection, B-factors, thermal stability, protein engineering, site-directed mutation, pullulanase, molecular
Rights & PermissionsPrintExport