Background: Cadmium is a major heavy metal pollutant. Even at low
concentrations in waste water Cadmium can accumulate in algae and sediments, and it
is absorbed by both plants and aquatic animals, like shellfish and fish. Triploid crucian
carp is an important economic fish, and have been farmed on a large scale in China.
Objective: to explore the molecular mechanisms underlying Cadmium stress of triploid
Method: we applied following experimental method to conduct research: transcriptomes
analysis, histological observation, Quantitative real-time PCR and enzyme activity
analysis et al.
Results: in the present study, we obtained following important results: 1) Under Cd
stress, the mortality and abnormality rates in triploid crucian carp were lower than those
of diploid strains and the effects of Cadmium treatment on the liver were revealed by
histological observation; 2) the liver transcriptomes of triploid crucian carp were obtained
and the data was analyzed; 3) 14 genes related to algae and sediments stress were
screened from transcriptome data, and the expression of these genes was verified by
Quantitative real-time PCR. The results were consistent with the gene results in
transcriptome data; 4) Quantitative real-time PCR and enzyme activity analysis all
confirmed triploid crucian carp resistance to Cadmium stress was regulated by both
oxidative stress and ER stress responses; 5) We found that IRE-1 and PERK, not ATF-
6, were involved in the enhance Cadmium resistance of triploid crucian carp.
Conclusion: these studies demonstrate that triploid crucian carp have strong resistance