Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

Author(s): Hassan Valadbeigi, Elham Esmaeeli, Sobhan Ghafourian, Abbas Maleki, Nourkhoda Sadeghifard*.

Journal Name: Infectious Disorders - Drug Targets
(Formerly Current Drug Targets - Infectious Disorders)

Volume 19 , Issue 3 , 2019

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Abstract:

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam.

Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes.

Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained.

Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

Keywords: UPEC, gene profile, antibiotic susceptibility, urinary tract, molecular analysis, E.coli, molecular analysis.

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Article Details

VOLUME: 19
ISSUE: 3
Year: 2019
Page: [322 - 326]
Pages: 5
DOI: 10.2174/1871526518666181113091322
Price: $58

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