Antimicrobial Activity, Antioxidant Properties and Phytochemical Screening of Aesculus hippocastanum Mother Tincture against Food-borne Bacteria

Author(s): Maria Grazia Bonomo*, Caterina Cafaro, Daniela Russo, Luana Calabrone, Luigi Milella, Carmela Saturnino, Anna Capasso, Giovanni Salzano.

Journal Name: Letters in Drug Design & Discovery

Volume 17 , Issue 1 , 2020

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background: The advantageous health effects of extracts from different types of plants have been known for centuries and the search for new natural extracts is very important at present.

Methods: In this study, the antioxidant and the antimicrobial activities of Aesculus hippocastanum mother tincture (TM) against a range of foodborne bacteria were investigated to determine the major components and the action spectrum and the antimicrobial efficacy of the extract.

Results: Results demonstrated a high antioxidant ability; total polyphenolic content was 506.8 ± 15.2mg GAE/100ml and the highest content was found for flavonoids. Moreover, TM demonstrated the antimicrobial activity against all tested bacteria and all Gram-negative bacteria were sensitive with an high antimicrobial activity. The inhibitory activity showed a moderate effect on the growth of 72.7% of strains in presence of different extract MIC.

Conclusion: The synergistic actions of bioactive compounds detected in the TM might be on the basis of the antioxidant and biological activities observed. These results can be applied in the pharmaceutical field and also in food preservation, alternative medicine and natural therapies.

Keywords: Aesculus hippocastanum mother tincture, polyphenols content, flavonoids content, tannins content, antimicrobial activity, MIC, antioxidant properties.

[1]
Bonomo, M.G.; Russo, D.; Cristiano, C.; Calabrone, L.; Di Tomaso, K.; Milella, L.; Salzano, G. Antimicrobial activity, antioxidant properties and phytochemical screening of Echinacea angustifolia, Fraxinus excelsior and Crataegus oxyacantha mother tinctures against food-borne bacteria. EC Microbiol., 2017, 7(5), 173-181.
[2]
Bonomo, M.G.; Salzano, G. Microbial diversity and dynamics of Pecorino di Filiano PDO, a traditional cheese of Basilicata region (Southern Italy). Int. J. Dairy Technol., 2012, 65(4), 531-541.
[http://dx.doi.org/10.1111/j.1471-0307.2012.00860.x]
[3]
Margină, D.; Olaru, O.T.; Ilie, M.; Gradinaru, D.; Gutu, C.; Voicu, S.; Dinischiotu, A.; Spandidos, D.A.; Tsatsakis, A.M. Assessment of the potential health benefits of certain total extracts from Vitis vinifera, Aesculus hyppocastanum and Curcuma longa. Exp. Ther. Med., 2015, 10, 1681-1688.
[http://dx.doi.org/10.3892/etm.2015.2724]
[4]
Guidetti, G.; Di Cerbo, A.; Giovazzino, A.; Rubino, V.; Palatucci, A.T.; Centenaro, S.; Fraccaroli, E.; Cortese, L.; Bonomo, M.G.; Ruggiero, G.; Canello, S.; Terrazzano, G. In vitro effects of some botanicals with anti-inflammatory and antitoxic activity. J. Immunol. Res., 2016, 1-11.
[http://dx.doi.org/10.1155/2016/5457010]
[5]
Bibi, Y.; Nisa, S.; Chaudhary, F.M.; Zia, M. Antibacterial activity of some selected medicinal plants of Pakistan. BMC Complement. Altern. Med., 2011, 11(52), 1-7.
[http://dx.doi.org/10.1186/1472-6882-11-52]
[6]
Gutierrez, J.; Barry-Ryan, C.; Bourke, P. The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. Int. J. Food Microbiol., 2008, 124, 91-97.
[http://dx.doi.org/10.1016/j.ijfoodmicro.2008.02.028]
[7]
Laciar, A.; Vaca Ruiz, M.L.; Carrizo Flores, R.; Saad, J.R. Antibacterial and antioxidant activities of the essential oil of Artemisia echegarayi Hieron. (Asteraceae). Rev. Argent. Microbiol., 2009, 41, 226-231.
[8]
Vašková, J.; Fejerčáková, A.; Mojžišová, G.; Vaško, L.; Patlevič, P. Antioxidant potential of Aesculus hippocastanum extract and escin against reactive oxygen and nitrogen species. Eur. Rev. Med. Pharmacol. Sci., 2015, 19, 879-886.
[9]
Bilia, A.R.; Eterno, F.; Bergonzi, M.C.; Mazzi, G.; Vincieri, F.F. Evaluation of the content and stability of the constituents of mother tinctures and tinctures: The case of Crataegus oxyacantha L. and Hieracium pilosella L. J. Pharm. Biomed. Anal., 2007, 44, 70-78.
[http://dx.doi.org/10.1016/j.jpba.2007.01.046]
[10]
Kapusta, I.; Janda, B.; Szajwaj, B.; Stochmal, A.; Piacente, S.; Pizza, C.; Franceschi, F.; Franz, C.; Oleszek, W. Flavonoids in horse chestnut (Aesculus hippocastanum) seeds and powdered waste water byproducts. J. Agric. Food Chem., 2007, 55, 8485-8490.
[http://dx.doi.org/10.1021/jf071709t]
[11]
M.; Matławska, I. Flavonoids from the flowers of Aesculus hippocastanum. Acta Pol. Pharm., 2011, 68403408 .
[12]
Russo, D.; Valentão, P.; Andrade, P.B.; Fernandez, E.C.; Milella, L. Evaluation of antioxidant, antidiabetic and anticholinesterase activities of Smallanthus sonchifolius landraces and correlation with their phytochemical profiles. Int. J. Mol. Sci., 2015, 16, 17696-17718.
[http://dx.doi.org/10.3390/ijms160817696]
[13]
Armentano, M.F.; Bisaccia, F.; Miglionico, R.; Russo, D.; Nolfi, N.; Carmosino, M.; Andrade, P.B.; Valentão, P.; Diop, M.S.; Milella, L. Antioxidant and proapoptotic activities of Sclerocarya birrea [(A. Rich.) Hochst.] methanolic root extract on the hepatocellular carcinoma cell line HepG2. BioMed Res. Int., 2015, 1-11.
[http://dx.doi.org/10.1155/2015/561589]
[14]
Russo, D.; Bonomo, M.G.; Salzano, G.; Martelli, G.; Milella, L. Nutraceutical properties of Citrus clementina juices. Pharmacologyonline, 2012, 1, 84-93.
[15]
Gul, M.Z.; Bhakshu, L.M.; Ahmad, F.; Kondapi, A.K.; Qureshi, I.A.; Ghazi, I.A. Evaluation of Abelmoschus moschatus extracts for antioxidant, free radical scavenging, antimicrobial and antiproliferative activities using in vitro assays. BMC Complement. Altern. Med., 2011, 11, 64.
[http://dx.doi.org/10.1186/1472-6882-11-64]
[16]
Luximon-Ramma, A.; Bahorun, T.; Soobrattee, M.A.; Aruoma, O.I. Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extracts of Cassia fistula. J. Agric. Food Chem., 2002, 50, 5042-5047.
[http://dx.doi.org/10.1021/jf0201172]
[17]
Chew, Y.L.; Lim, Y.Y.; Omar, M.; Khoo, K.S. Antioxidant activity of three edible seaweeds from two areas in South East Asia. Lebensm. Wiss. Technol., 2008, 41, 1067-1072.
[http://dx.doi.org/10.1016/j.lwt.2007.06.013]
[18]
Malencic, D.; Maksimovic, Z.; Popovic, M.J. Miladinovic polyphenol contents and antioxidant activity of soybean seed extract. Bioresour. Technol., 2008, 99, 6688-6691.
[http://dx.doi.org/10.1016/j.biortech.2007.11.040]
[19]
Adeolu, A.A.; Florence, O.J.; Anthony, J.A.; Patrick, J.M. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complement. Altern. Med., 2008, 8, 54.
[http://dx.doi.org/10.1186/1472-6882-8-54]
[20]
Abdalbasit, A.M.; Ramlah, M.I.; Maznah, I.; Norsharina, I. Antioxidant activity and phenolic content of phenolic rich fractions obtained from black cumin (Nigella sativa) seedcake. Food Chem., 2009, 116, 306-312.
[http://dx.doi.org/10.1016/j.foodchem.2009.02.051]
[21]
Ke-Xue, Z.; Cai-Xia, L.; Xiao-Na, G.; Wei, P.; Hui-Ming, Z. Antioxidant activities and total phenolic contents of various extracts from defatted wheat germ. Food Chem., 2011, 126, 1122-1126.
[http://dx.doi.org/10.1016/j.foodchem.2010.11.144]
[22]
Kimura, H.; Ogawa, S.; Ishihara, T.; Maruoka, M.; Tokuyama-Nakai, S.; Jisaka, M.; Yokoa, K. Antioxidant activities and structural characterization of flavonol O-glycosides from seeds of Japanese horse chestnut (Aesculus turbinata BLUME). Food Chem., 2017, 228, 348-355.
[http://dx.doi.org/10.1016/j.foodchem.2017.01.084]
[23]
Bylka, W.; Matlawska, I.; Pilewski, N.A. Natural flavonoids as antimicrobial agents. J. American Nutr. Assoc., 2004, 7, 24-31.
[24]
Kostic, D.A.; Velickovic, J.M.; Mitic, S.S.; Mitic, M.N.; Randelovic, S.S. Phenolic content, and antioxidant and antimicrobial activities of Crataegus oxyacantha L (Rosaceae) fruit extract from Southeast Serbia. Trop. J. Pharm. Res., 2012, 11(1), 117-124.
[http://dx.doi.org/10.4314/tjpr.v11i1.15]


Rights & PermissionsPrintExport Cite as


Article Details

VOLUME: 17
ISSUE: 1
Year: 2020
Page: [48 - 56]
Pages: 9
DOI: 10.2174/1570180816666181108114115
Price: $65

Article Metrics

PDF: 17
HTML: 2