Background: The advantageous health effects of extracts from different types of plants
have been known for centuries and the search for new natural extracts is very important at present.
Methods: In this study, the antioxidant and the antimicrobial activities of Aesculus hippocastanum
mother tincture (TM) against a range of foodborne bacteria were investigated to determine the major
components and the action spectrum and the antimicrobial efficacy of the extract.
Results: Results demonstrated a high antioxidant ability; total polyphenolic content was
506.8±15.2mg GAE/100ml and the highest content was found for flavonoids. Moreover, TM
demonstrated the antimicrobial activity against all tested bacteria and all Gram-negative bacteria
were sensitive with an high antimicrobial activity. The inhibitory activity showed a moderate effect
on the growth of 72.7% of strains in presence of different extract MIC.
Conclusion: The synergistic actions of bioactive compounds detected in the TM might be on the
basis of the antioxidant and biological activities observed. These results can be applied in the
pharmaceutical field and also in food preservation, alternative medicine and natural therapies.