Background: Though after a start of genome sequencing most of the protein sequences
are deposited in databases, some proteins remain to be unannotated and functionally
uncharacterized. Chlamydia trachomatis L2C is a gram-negative pathogen bacterium involved in
causing severe disorders like lymphogranuloma venereum, nongonococcal urethritis, and
Objectives: Analyzing and annotating the hypothetical proteins can help to understand its
pathogenicity and therapeutic hotspots. Its genome encodes a total of 221 hypothetical proteins and
out of these, 14 hypothetical proteins are declared as virulent by virulence prediction server
Methods: In this study, the functional and structural analysis was carried out by conserve domain
finding servers, protein function annotators and physiochemical properties predictors. Proteinprotein
interactions studies revealed the involvement of these virulent HPs in a number of
pathways, which would be of interest for drug designers.
Results: Classifier tool was used to classify the virulent hypothetical proteins into enzymes,
membrane protein, transporter and regulatory protein groups.
Conclusion: Our study would help to understand the mechanisms of pathogenesis and new
potential therapeutic targets for a couple of diseases caused by C. trachomatis.