Background: Tumors of the childhood are considered to be grave and devastating pathologies,
with high mortality rates. Current therapeutic options like cytotoxic drugs and radiotherapy
target both healthy and malignant cells, thus resulting in long-term neurological and intellectual
sequelae and endocrinological disorders.
Objectives: In this study, we focused on the anticancer potency of crocins, the main constituents
of Crocus sativus L, stigmas. Crocins were first extracted using organic solvents from the dried
stigmas and then were identified using the HPLC analysis.
Materials and Methods: TE-671 cells were treated with the extract of crocins using a range of
concentrations between 0.25-mg/ mL and 16 mg/mL. Viability of the cells was measured at 24h,
48h, 72h and 96h. In addition, we have examined the expression levels of the p53 gene using
Real-Time Reverse Transcription PCR.
Results: Results showed that crocins exerted significant cytotoxic and anti-proliferative effects in
a concentration and time - dependent-manner on TE-671 cells. Furthermore, p53 manifested similar
expression pattern as the anti-proliferative effect of crocin.
Conclusion: Our data demonstrate that crocins could be a novel promising agent for the improvement
of tumor treatment.
Keywords: saffron, crocins, cytotoxicity, anti-proliferation, medulloblastoma, CNS tumors, TE671
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