Chemical Constituents Isolated from Rhododendron ungernii with Antioxidant Profile

Author(s): Emine Dede, Nusret Genc, Mahfuz Elmastas, Huseyin Aksit, Ramazan Erenler*.

Journal Name: The Natural Products Journal

Volume 9 , Issue 3 , 2019

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Graphical Abstract:


Abstract:

Background: Plant in Rhododendron genus that contains phenolic compounds has been used in traditional medicine and revealed considerable biological activities.

Objective: Isolation and identification of antioxidant natural products from Rhododendron ungernii.

Methods: Rhododendron ungernii Trautv. flowers were collected and dried in shade. The dried flowers were extracted with methanol for 3 days. The solvent was removed by reduced pressure to yield the extract which was subjected to column chromatography (Sephadex LH-20, C18 reversed phase column) to isolate catechin-7-O-glucoside (1), quercetin-3-O-β-galactoside (2), quercetin-3-O- β-xyloside (3), farrerol (4), myricetin (5), and quercetin (6). The structures of isolated compounds were elucidated by spectroscopic methods such as 1D-NMR, 2D-NMR, and LC-TOF/MS. DPPH• scavenging effect, ABTS•+ scavenging activity, and reducing power (FRAP) were performed for antioxidant assays of isolated natural compounds.

Results: Isolated flavonoids displayed the outstanding antioxidant activities. Catechin-7-O-glucoside (1) and quercetin-3-O-β-galactoside (2) (IC50, 3.66 µg/mL) had the most DPPH• scavenging effect among the compounds. The highest ABTS•+ scavenging activity (IC50, 1.41 µg/mL) and reducing power effect (6.05 mmol TE/g comp) were observed for myricetin (5).

Conclusion: R. ungernii extract and isolated compounds could be a promising antioxidant for food and pharmaceutical industries.

Keywords: Rhododendron ungernii, antioxidant, active compounds, flavonoids, phenolic compounds, biological activities.

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Article Details

VOLUME: 9
ISSUE: 3
Year: 2019
Page: [238 - 243]
Pages: 6
DOI: 10.2174/2210315508666181024114812
Price: $58

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