Background: Delivery of foreign cargoes into cells is of great value for bioengineering research
and therapeutic applications.
Objective: In this study, we proposed and established a carrier-free gene delivery platform utilizing
staggered herringbone channel and silicon nanoneedle array, to achieve high-throughput in vitro gene
Methods: With this microchip, fluidic micro vortices could be induced by the staggered-herringboneshaped
grooves within the channel, which increased the contact frequency of the cells with the channel
substrate. Transient disruptions on the cell membrane were well established by the nanoneedle array on
Result: Compared to the conventional nanoneedle-based delivery system, proposed microfluidic chip
achieved flow-through treatment with high gene transfection efficiency (higher than 20%) and ideal cell
viability (higher than 95%).
Conclusion: It provides a continuous processing environment that can satisfy the transfection requirement
of large amounts of biological molecules, showing high potential and promising prospect for both
basic research and clinical application.