A series of 5-(2-amino-6-(3/4-bromophenyl)pyrimidin-4-yl)benzene-1,3-diol scaffolds was
synthesized by Claisen-Schmidt condensation and characterized by NMR, IR, Mass and elemental
analyses. The synthesized pyrimidine scaffolds were screened for their antimicrobial activity by tube
dilution method as well for antiproliferative activity (human colorectal (HCT116) cancer cell line) by
SRB assay. The antimicrobial screening results demonstrated that compounds, k6, k12, k14 and k20
were found to be the most potent ones against selected microbial species. The anticancer screening results
indicated that compounds, k8 and k14 displayed potent anticancer activity against cancer cell line
(HCT116). Further, the molecular docking study carried to find out the interaction between active
pyrimidine compounds with CDK-8 protein indicated that compound k14 showed best dock score with
better potency within the ATP binding pocket and may be used as a lead for rational drug designing of
the anticancer molecule.
Keywords: Antibacterial activity, antifungal activity, docking, cytotoxicity, mechanism, pyrimidine scaffolds, synthesis.
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