Background: 2-Aminochromene derivatives display important pharmacological properties,
including mainly antibiotic and anticancer activities.
Objective: The study aims to synthesize new chromene derivatives via a new approach using Grignard
reagents, for the evaluation of their antibiotic and antifungal properties.
Method: A series of novel 3-cyano-4-aminochromene derivatives bearing alkyl substituents at the
4-position was prepared for biological evaluation.
Results: These compounds were obtained by the addition of various Grignard reagents into Nethoxycarbonyl-
3-cyanoiminocoumarines in moderate to good yields (72-96%). The reaction is
completely regioselective. The new chromene derivatives were screened for their in vitro antimicrobial
activities against a panel of six bacterial and three fungal strains using agar dilution
Conclusion: The antibacterial activity of the chromene derivatives was more pronounced on
Gram-positive bacteria than on Gram-negative bacteria with a significant activity observed against
Staphylococcus aureus. An interesting antifungal activity against Fusarium sp. and Fusarium
oxysporum was also noticed.