Background: Macrolide antibiotics are known as versatile broad-spectrum antibiotics.
Macrolides belong to the oldest group of antibacterial agents. The macrolides which are frequently
used for clinical purposes are broadly categorized in three classes depending on the number of
membered macrocyclic lactone ring. These three classes are actually consisting 14, 15 or 16 atoms in
macrocyclic lactone ring which are linked through glycosidic bonds. Erythromycin, azithromycin clarithromycin
and roxithromycin are frequently used to control against bacterial infections.
Methods: The quality assurance and quality controls are important tasks in the pharmaceutical industries.
Consequently, to check the quality of drugs, there is a strong need to know about alternative
analytical methods for the routine analysis. Many methods have been reported in the literature for the
quantitative determination of erythromycin, clarithromycin, azithromycin and clarithromycin in
pharmaceutical formulations and biological samples.
Results: This review will cover a brief introduction of erythromycin, azithromycin, clarithromycin and
roxithromycin as well as analytical techniques for their assessment. Each developed method has its
own merits and demerits.
Conclusion: Any accurate method could be used for the quality control and quality assurance of
macrolide antibiotics according to the availability, performance and procedure of selected instrument
as well as skill and expertise of the analyst.