Optimization of Process Parameters for Production of Pectinase using Bacillus Subtilis MF447840.1

Author(s): Ram Balak Mahto*, Mukesh Yadav, Soumya Sasmal, Biswnath Bhunia.

Journal Name: Recent Patents on Biotechnology

Volume 13 , Issue 1 , 2019

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background: Pectinase enzyme has immense industrial prospects in the food and beverage industries.

Objective: In our investigation, we find out the optimum process parameters suitable for better pectinase generation by Bacillus subtilis MF447840.1 using submerged fermentation.

Method: 2% (OD600 nm = 0.2) of pure Bacillus subtilis MF447840.1 bacterial culture was inoculated in sterile product production media. The production media components used for this study were 1 g/l of pectin, 2 g/l of (NH4)2SO4, 1 g/l of NaCl, 0.25 g/l of K2HPO4, 0.25 g/l of KH2PO4 and 1 g/l of MgSO4 for pectinase generation. We reviewed all recent patents on pectinase production and utilization. The various process parameters were observed by changing one variable time method.

Results: The optimum fermentation condition of different parameters was noticed to be 5% inoculums, 25% volume ratio, temperature (37°C), pH (7.4) and agitation rate (120 rpm) following 4 days incubation.

Conclusion: Maximum pectinase generation was noticed as 345 ± 12.35 U following 4 days incubation.

Keywords: Pectinase, Bacillus, optimization, process parameter, enzyme, pectic substances.

[1]
McLellan MR, Kime RW, Lind LR. Apple juice clarification with the use of honey and pectinase. J Food Sci 1985; 50(1): 206-8.
[2]
Hinnergardt LC, Szalkucki P, Sando ML. Process for preparing a tomato sauce using a pectinase enzyme. US5,902,616, 1999.
[3]
Sanderson G, Simpson W. Pectinase enzyme treating process for preparing high bulk density tea powders. US3,787,582, 1974.
[4]
Jean PR. Viscosity controlled pectinolytic enzyme addition to fruit and vegetable juice. US3,795,521, 1974.
[5]
Thornton JW, Eckerman CS, Ekman RO, Holmbom BR. Treatment of alkaline bleached mechanical wood pulp with pectinase. US5,487,812, 1996.
[6]
Olsen WL, Zhu H, Hubbe MA. Method of improving pulp freeness using cellulase and pectinase enzymes. US6,066,233, 2000.
[7]
Kumar S, Jain NK, Sharma KC, et al. Optimization, purification and characterization of pectinases from pectinolytic strain, Aspergillus foetidus MTCC 10559. J Environ Biol 2015; 36(2): 483-9.
[8]
Sahay S, Hamid B, Singh P, et al. Evaluation of pectinolytic activities for oenological uses from psychrotrophic yeasts. Lett Appl Microbiol 2013; 57(2): 115-21.
[9]
Nakagawa T, Nagaoka T, Taniguchi S, Miyaji T, Tomizuka N. Isolation and characterization of psychrophilic yeasts producing cold-adapted pecti-nolytic enzymes. Lett Appl Microbiol 2004; 38(5): 383-7.
[10]
Bhunia B, Basak B, Bhattacharya P, Dey A. Kinetic studies of alkaline protease from Bacillus licheniformis NCIM-2042. J Microbiol Biotechnol 2012; 22(12): 1758-66.
[11]
Bhunia B, Dutta D, Chaudhuri S. Selection of suitable carbon, nitrogen and sulphate source for the production of alkaline protease by Bacillus licheniformis NCIM-2042. Notulae Scientia Biologicae 2010; 2(2): 56-9.
[12]
Bhunia B, Basak B, Dey A. A review on production of serine alkaline protease by Bacillus spp. J Biochem Technol 2012; 3(4): 448-57.
[13]
Bhunia B, Behera KK, Baquee A, Sharma HP. Optimization of alkaline protease activity from Bacillus subtilis 2724 by response surface methodology (RSM). Int J Biol Sci Eng 2010; 1: 158-69.
[14]
Bhunia B, Dutta D, Chaudhuri S. Extracellular alkaline protease from Bacillus licheniformis NCIM‐2042: Improving enzyme activity assay and characterization. Eng Life Sci 2011; 11(2): 207-15.
[15]
Ying-xian Y. The status quo and strategy of hemp bast degumming. Shandong Textile Sci Technol 2005; 1: 51-4.
[16]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54.
[17]
Lakshmi GS, Rao CS, Rao RS, Hobbs PJ, Prakasham RS. Enhanced production of xylanase by a newly isolated Aspergillus terreus under solid state fermentation using palm industrial waste: A statistical optimization. Biochem Eng J 2009; 48: 51-7.
[18]
Shuler ML, Kargi F. Bioprocess Engineering: Basic Concept. Prentice Hall of India Private Limited, New Delhi 2008.
[19]
Phisalaphong M, Srirattana N, Tanthapanichakoon W. Mathematical modeling to investigate temperature effect on kinetic parameters of ethanol fermentation. Biochem Eng J 2006; 28: 36-43.
[20]
Bhunia B, Basak B, Bhattacharya P, Dey A. Process engineering studies to investigate the effect of temperature and pH on kinetic parameters of alkaline protease production. J Bioscience Bioeng 2013; 115(1): 86-9.


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 13
ISSUE: 1
Year: 2019
Page: [69 - 73]
Pages: 5
DOI: 10.2174/1872208312666180917094428
Price: $58

Article Metrics

PDF: 22
HTML: 2
EPUB: 1
PRC: 1