Background: Voriconazole (VORI), a derivative of fluconazole, shows a greater selectivity
for the fungal enzyme than for the corresponding rat liver enzyme as compared to Ketoconazole
Methods: An environment friendly, simple, rapid, and isocratic robust supercritical fluid chromatographic
method has been developed and validated for determining Voriconazole (VORI) in Bulk
drug and laboratory mixture representing the marketed dosage form. Efficient chromatographic
separation was achieved on a SFC Packed Crest C18 T (150 × 4.0 mm, 5.0 µm) with mobile phase
CO2, in isocratic combination with methanol (MeOH) 90: 10, respectively, at a flow rate of 1.0
mL/min; the eluent being monitored at 256 nm using UV-detector. The supercritical nature of carbon
dioxide was maintained at 12 MPa pressure and 40°C temperature.
Results: In the optimized method, the retention time of VORI was 3.5 minute. The test solution was
found to be stable in the blank for 24 h. Robustness of the developed SFC method was optimized
by varying chromatographic conditions viz. column oven temperature, flow rate, SFC temperature
and pressure change.
Conclusion: Regression analyses indicate correlation coefficient value greater than 0.997 for VORI
in the linearity range of 100-400 µg/mL. The method had shown good, consistent recoveries for
VORI (99.3-101.2%). The method was found to be accurate, precise, linear, specific, sensitive,
rugged and robust.