quinolinone (vesnarinone), a novel inotropic drug with unique and complex mechanisms of
action, is known to show antitumor activity against several human malignancies. In the present
study, vesnarinone-induced signal transduction of S-phase kinase-associated protein 2 (Skp2) and
nuclear factor-kappa beta (NF-kB) as molecular targets of oral malignant Burkitt’s lymphoma
(Raji cells) was evaluated.
Methods: Raji cells were incubated with vesnarinone at concentrations of 0, 1.25x10-2, 2.50x10-
2, or 5.0x10-2 Molar. After 24 h, chemotactic cell migration was examined by a Boyden chamber
kit. Apoptosis induction was observed by caspase-9 colorimetric assay. To evaluate levels of
Skp2, NF-kB, and κ-tubulin, Western blot analysis was performed.
Results: Vesnarinone markedly suppressed chemotactic cell migration and significantly induced
apoptosis by increasing the caspase-9 activity of Raji cells through down regulation of Skp2
Conclusion: Vesnarinone decreased the expression of Skp2 and NF-kB indicating that these
molecules may be targeted for the treatment of oral malignant Burkitt’s lymphoma (BL). The
results of this work offer a promising therapeutic approach for BL tumors.