Background: Insulin resistance is a frequent metabolic disorder in Polycystic
Ovary Syndrome (PCOS). Moringa oleifera has been shown to increase insulin expression
and decrease the degree of insulin in diabetes mellitus, therefore it is expected that
Moringa oleifera could decrease insulin levels and increase folliculogenesis in PCOS.
Objective: To prove the effect of Moringa oleifera leaf extract in various doses might decrease
the insulin levels and increase folliculogenesis in female PCOS-insulin resistant
Methods: The three month old white rat of Wistar strain (Rattus norvegicus) 100-130
grams were divided into five groups (n=8) including normal control, PCOS-insulin resistance,
PCOS-insulin resistance given metformin and PCOS-resistance insulin were given
Moringa oleifera leaf extract in two doses. Then, the PCOS model-insulin resistance by
injection of testosterone propionate for 28 days. After 14 days treatment, we analysed insulin
levels and folliculogenesis.
Results: The PCOS control group showed a significant increase in insulin levels compared
to the normal control group. The insulin levels from group treatment with Moringa
oleifera leaf extract of 250 mg/kgBW was significantly lower than the PCOS control
group. Ovarian histology analysis found that the number and diameter of follicle of
PCOS control group showed a significant decrease compared to normal control group. In
addition, the treatment with metformin and leaf Moringa oleifera dose 250 mg/kgBW and
500 mg/kgBW showed significant increase of folliculogenesis compared to PCOS control
Conclusions: Moringa oleifera could lowering the blood insulin levels, subsequently
decreasing the androgen thus allowed the increasing of folliculogenesis in PCOS.