Isolation and Molecular Identification of Aeromonas Wound Infection in Iranian Burn Patients

Author(s): Nahal Hadi*, Zhaleh Mahmoodi, Amir Emami, Yalda Malekzadegan, Tahereh Valadbeygi.

Journal Name: Infectious Disorders - Drug Targets
(Formerly Current Drug Targets - Infectious Disorders)

Volume 19 , Issue 3 , 2019

Become EABM
Become Reviewer

Graphical Abstract:


Introduction: Aeromonas have recently emerged as opportunistic pathogens and only a few studies are available regarding the isolation of these bacteria from burn wound infections. This study aimed at isolating Aeromonas as an infrequent cause of infection in this group of immunocompromised patients.

Methods: A total of 300 samples were collected from the wounds of burn patients hospitalized in Gotbodin Shirazi Burn Center in 2013. The samples were cultured on Aeromonas specific media and then confirmed using standard biochemical tests and 16S rRNA gene amplification and sequencing. The antibiotic susceptibility of the isolates was determined using the disk diffusion and broth microdilution methods.

Results: Biochemical tests demonstrated five presumptive samples of Aeromonas, while, molecular testing confirmed only three. All isolates were resistant to ampicillin-sulbactam, erythromycin, oxacillin, and vancomycin. However, they were susceptible to gentamicin, meropenem, nitrofurantoin, chloramphenicol, cephalexin, and cefotaxime. Two Aeromonas veronii isolates were resistant to sulfamethoxazole-trimethoprim and sensitive to nalidixic acid, while Aeromonas bestiarum was sensitive to the former and resistant to the latter antibiotics. Lastly, only one of the A. veronii isolates was found to be resistant to tetracycline.

Conclusion: This study is the first to report on the isolation of Aeromonas spp. from burn patients in Iran. Moreover, this is the first report of isolating A. bestiarum from burn wounds. The results of this study confirm earlier findings that the use of molecular methods is essential to accurately identify these bacteria.

Keywords: Aeromonas, burn wound, nosocomial infections, 16S rRNA, wound infection, patients.

Mukhopadhyay, C.; Chawla, K.; Sharma, Y.; Bairy, I. Emerging extra-intestinal infections with Aeromonas hydrophila in coastal region of southern Karnataka. J. Postgrad. Med., 2008, 54(3), 199-202.
[] [PMID: 18626167]
Igbinosa, I.I.E.; Aghdasi, F.; Tom, M.; Okoh, A. Emerging aeromonas species infections and their significance in public health. ScientificWorldJournal, 2012, 2012(1), 1-13.
Ghenghesh, K.S.; Ahmed, S.F.; El-Khalek, R.A.; Al-Gendy, A.; Klena, J. Aeromonas-associated infections in developing countries. J. Infect. Dev. Ctries., 2008, 2(2), 81-98.
[] [PMID: 19738331]
Surek, M.; Vizzotto, B.S.; Souza, E.M. Pedrosa, Fde.O.; Dallagassa, C.B.; Farah, S.M.; Fadel-Picheth, C.M. Identification and antimicrobial susceptibility of Aeromonas spp. isolated from stool samples of Brazilian subjects with diarrhoea and healthy controls. J. Med. Microbiol., 2010, 59(Pt 3), 373-374.
[] [PMID: 19942692]
Parker, J.L.; Shaw, J.G. Aeromonas spp. clinical microbiology and disease. J. Infect., 2011, 62(2), 109-118.
[] [PMID: 21163298]
Yu, H.B.; Zhang, Y.L.; Lau, Y.L.; Yao, F.; Vilches, S.; Merino, S.; Tomas, J.M.; Howard, S.P.; Leung, K.Y. Identification and characterization of putative virulence genes and gene clusters in Aeromonas hydrophila PPD134/91. Appl. Environ. Microbiol., 2005, 71(8), 4469-4477.
[] [PMID: 16085838]
Hadi, N.; Yang, Q.; Barnett, T.C.; Tabei, S.M.; Kirov, S.M.; Shaw, J.G. Bundle-forming pilus locus of Aeromonas veronii bv. Sobria. Infect. Immun., 2012, 80(4), 1351-1360.
[] [PMID: 22311923]
Dias, C.M.V.; Martinez-Murcia, A.; Saavedra, M.J. Antimicrobial resistance patterns of aeromonas spp. isolated from ornamenta fish. J. Aquac. Res. Dev., 2012, 3(3), 1-4.
Jun, J.K.J.; Gomez, D. Occurrence of tetracycline-resistant Aeromonas hydrophila infection in Korean cyprinid loach. Afr. J. Microbiol. Res., 2010, 4(9), 849-855.
Ebrahim-Saraie, H.S.; Nezhad, N.Z.; Heidari, H.; Motamedifar, A.; Motamedifar, M. Detection of antimicrobial susceptibility and integrons among extended-spectrum β-lactamase producing uropathogenic Escherichia coli Isolates in Southwestern Iran. Oman Med. J., 2018, 33(3), 218-223.
[] [PMID: 29896329]
Soltani, B.; Heidari, H.; Ebrahim-Saraie, H.S.; Hadi, N.; Mardaneh, J.; Motamedifar, M. Molecular characteristics of multiple and extensive drug-resistant Acinetobacter baumannii isolates obtained from hospitalized patients in Southwestern Iran. Infez. Med., 2018, 26(1), 67-76.
[PMID: 29525800]
Emami, S.; Nikokar, I.; Ghasemi, Y.; Ebrahimpour, M.; Sedigh Ebrahim-Saraie, H.; Araghian, A.; Faezi, S.; Farahbakhsh, M.; Rajabi, A. Antibiotic resistance pattern and distribution of psla gene among biofilm producing pseudomonas aeruginosa isolated from waste water of a burn center. Jundishapur J. Microbiol., 2015, 8(11)e23669
[] [PMID: 26855739]
Malekzadegan, Y.; Hadadi, M.; Ebrahim-Saraie, H.S.; Heidari, H.; Motamedifar, M. Antimicrobial resistance pattern and frequency of multiple-drug resistant enterobacter spp. at a tertiary care hospital in southwest of Iran. J Krishna Inst Med Sci, 2017, 6(2), 33-39.
Yang, J.L.; Wang, M.S.; Cheng, A.C.; Pan, K.C.; Li, C.F.; Deng, S.X. A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection. World J. Gastroenterol., 2008, 14(18), 2872-2876.
[] [PMID: 18473413]
Lee, C.; Cho, J.C.; Lee, S.H.; Lee, D.G.; Kim, S.J. Distribution of Aeromonas spp. as identified by 16S rDNA restriction fragment length polymorphism analysis in a trout farm. J. Appl. Microbiol., 2002, 93(6), 976-985.
[] [PMID: 12452953]
Kienzle, N.; Muller, M.; Pegg, S. Aeromonas wound infection in burns. Burns, 2000, 26(5), 478-482.
[] [PMID: 10812272]
Skoll, P.J.; Hudson, D.A.; Simpson, J.A. Aeromonas hydrophila in burn patients. Burns, 1998, 24(4), 350-353.
[] [PMID: 9688201]
Persson, S.; Al-Shuweli, S.; Yapici, S.; Jensen, J.N.; Olsen, K.E. Identification of clinical aeromonas species by rpoB and gyrB sequencing and development of a multiplex PCR method for detection of Aeromonas hydrophila, A. caviae, A. veronii, and A. media. J. Clin. Microbiol., 2015, 53(2), 653-656.
[] [PMID: 25411168]
Yasti, A.C.; Otan, E.; Doganay, M.; Kama, N.A. Aeromonas hydrophila in a burn patient. J. Burn Care Res., 2009, 30(5), 898-900.
[] [PMID: 19692919]
Chim, H.; Song, C. Aeromonas infection in critically ill burn patients. Burns, 2007, 33(6), 756-759.
[] [PMID: 17521817]
Barillo, D.J.; McManus, A.T.; Cioffi, W.G.; McManus, W.F.; Kim, S.H.; Pruitt, B.A., Jr Aeromonas bacteraemia in burn patients. Burns, 1996, 22(1), 48-52.
[] [PMID: 8719317]
Lai, C.C.; Shiao, C.C.; Lu, G.D.; Ding, L.W. Aeromonas hydrophila and Aeromonas sobria bacteremia: rare pathogens of infection in a burn patient. Burns, 2007, 33(2), 255-257.
[] [PMID: 17084982]
Beaz-Hidalgo, R.; Latif-Eugenín, F.; Hossain, M.J.; Berg, K.; Niemi, R.M.; Rapala, J.; Lyra, C.; Liles, M.R.; Figueras, M.J. Aeromonas aquatica sp. nov., Aeromonas finlandiensis sp. nov. and Aeromonas lacus sp. nov. isolated from Finnish waters associated with cyanobacterial blooms. Syst. Appl. Microbiol., 2015, 38(3), 161-168.
[] [PMID: 25852023]
Küpfer, M.; Kuhnert, P.; Korczak, B.M.; Peduzzi, R.; Demarta, A. Genetic relationships of Aeromonas strains inferred from 16S rRNA, gyrB and rpoB gene sequences. Int. J. Syst. Evol. Microbiol., 2006, 56(Pt 12), 2743-2751.
[] [PMID: 17158971]
Minana-Galbis, D.F.M.; Fuste, M.C.; Loren, J.G. Genetic diversity and population gestalt of Aeromonas hydrophila, Aeromonas bestiarum, Aeromonas salmonicida and Aeromonas popoffii by multilocus enzyme electrophoresis (MLEE). Environ. Microbiol., 2004, 6(3), 198-208.
[] [PMID: 14871204]
Yáñez, M.A.; Catalán, V.; Apráiz, D.; Figueras, M.J.; Martínez-Murcia, A.J. Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequences. Int. J. Syst. Evol. Microbiol., 2003, 53(Pt 3), 875-883.
[] [PMID: 12807216]

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2019
Page: [269 - 273]
Pages: 5
DOI: 10.2174/1871526518666180903165823
Price: $58

Article Metrics

PDF: 30