Introduction: Aeromonas have recently emerged as opportunistic pathogens and only a
few studies are available regarding the isolation of these bacteria from burn wound infections.
This study aimed at isolating Aeromonas as an infrequent cause of infection in this group of immunocompromised
Methods: A total of 300 samples were collected from the wounds of burn patients hospitalized in
Gotbodin Shirazi Burn Center in 2013. The samples were cultured on Aeromonas specific media
and then confirmed using standard biochemical tests and 16S rRNA gene amplification and sequencing.
The antibiotic susceptibility of the isolates was determined using the disk diffusion and
broth microdilution methods.
Results: Biochemical tests demonstrated five presumptive samples of Aeromonas, while, molecular
testing confirmed only three. All isolates were resistant to ampicillin-sulbactam, erythromycin,
oxacillin, and vancomycin. However, they were susceptible to gentamicin, meropenem, nitrofurantoin,
chloramphenicol, cephalexin, and cefotaxime. Two Aeromonas veronii isolates were
resistant to sulfamethoxazole-trimethoprim and sensitive to nalidixic acid, while Aeromonas
bestiarum was sensitive to the former and resistant to the latter antibiotics. Lastly, only one of the
A. veronii isolates was found to be resistant to tetracycline.
Conclusion: This study is the first to report on the isolation of Aeromonas spp. from burn patients
in Iran. Moreover, this is the first report of isolating A. bestiarum from burn wounds. The results
of this study confirm earlier findings that the use of molecular methods is essential to accurately
identify these bacteria.