Background: Various analogues of benzimidazole are found to be biologically and therapeutically
potent against several ailments. Benzimidazole when attached with heterocyclic rings has
shown wide range of potential activities. So, from the above provided facts, we altered benzimidazole
derivatives so that more potent antagonists could be developed. In the search for a new category of antimicrobial
and anticancer agents, novel azomethine of 2-mercaptobenzimidazole derived from 3-(2-
(1H-benzo[d]imidazol-2-ylthio)acetamido)benzohydrazide were synthesized.
Results and Discussion: The synthesized analogues were characterized by FT-IR, 1H/13C-NMR and
MS studies as well C, H, N analysis. All synthesized compounds were evaluated for in vitro antibacterial
activities against Gram-positive (B. subtilis), Gram-negative (E. coli, P. aeruginosa, K. pneumoniae and
S. typhi) strains and in vitro antifungal activity against Candida albicans and Aspergillus niger strains
by serial dilution method, the minimum inhibitory concentration (MIC) described in μM/ml. The in
vitro anticancer activity of synthesized compounds was determined against humancolorectal carcinoma
cell line (HCT-116) using 5-fluorouracil as standard drug.
Conclusion: In general, all the synthesized derivatives exhibited significant antimicrobial and anticancer
activities. Compounds 8, 10, 15, 16, 17, 20 and 22 showed significant antimicrobial activity towards
tested bacterial and fungal strains and compound 26 exhibited significant anticancer activity.