Background: Besides the first-line medication for the treatment of type 2 diabetes, the
growth inhibitory activity of metformin alone or in combination with conventional chemotherapeutics
has been addressed on a panel of cell lines. In this study, we investigated the cytotoxicity and apoptosis
of the metformin alone and in combination with dacarbazine in Raji and Ramos lymphoma cell
Methods: Cell viability and apoptosis measured using resazurin assay, flow cytometry of PI stained
cells and western blot analysis.
Results: Metformin showed synergistic cytotoxic effects in combination with dacarbazine, reduced
cell viability, and increased apoptosis in Raji and Ramos lymphoma cells in comparison with the use
of each drug alone. The activation of MAPK and SAPK/JNK (P-SAPK/JNK) were shown in both
Conclusion: Overall, the result verified the synergistic effect of the metformin-dacarbazine combination,
which has the value in reducing the chemotherapeutic agent dose, adverse effect and the burden
of treatment for the community and patients.