Background and Objective: Today, resistance to multiple classes of antibiotics,
and notably to the β-lactam and aminoglycosides in A. baumannii is becoming a great problem
and it necessitates to make a new approach to combat with multidrug-resistant (MDR),
extensive drug-resistance (XDR) or Pandrug-resistant (PDR) isolates. In this case, a new
strategy and ways should be designed and introduced against such infections. Therefore the
aim of the present study was the evaluation of antibacterial activity of nanoconjugate gentamicin
and amikacin with gold against clinical isolates of A. baumannii that were collected
from burn wound infection. There are some patents of gold nanoparticles that are conjugated
with antibiotics (WO2017161296A1, US20090181101A1).
Methods: Eighteen A. baumannii were collected from burn wound infections. For confirmation
and detection of aminoglycoside-resistant genes, PCR was carried out. Gold nanoparticles
and nanoconjugates were prepared according to the protocol. For the evaluation of the
nanoconjugate, Dynamic light cattering, Transmission electron microscopy and FTIR Analysis
were carried out. Then, the antibacterial activity of nanoconjugates was conducted by
using micro broth dilution method.
Result: Prevalence of aminoglycoside-resistant genes was aacC1, aphA6, aadA1, aadB genes
55.5%, 22.2%, 38.8% and 22.2% respectively. Synthesis of bare nanoconjugates resulted in
nanoparticle in a size of 10 nm. Amikacin bound to Gnps showed excellent antibacterial activity
(94.5%) and just one isolate showed intermediate resistance. Also, gentamicin bound to
Gnps had a good antimicrobial effect (50%) in contrast to gentamicin alone.
Conclusion: Our study showed that a combination of amikacin and gentamicin with Gnps
has a significant antibacterial efficiency against clinical isolates of A. baumannii. Gnps can
be used as extraordinary molecular carriers for targeting, and delivery of the antibiotic molecules
to the specific infection.