Preparation of Molecularly Imprinted SPR Nanosensor for Myoglobin Detection

Author(s): Nevra Ozturk Atay, Bilgen Osman, Sinan Akgol, Alev Karagozler, Adil Denizli*.

Journal Name: Current Applied Polymer Science

Volume 2 , Issue 2 , 2018

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Abstract:

Background: As it is released into the blood during the early stages of myocardial infarction (MI), myoglobin is one of the best cardiac markers. Its concentration in the blood increases shortly after MI and reaches a maximum about 12 h after the onset of MI symptoms. These features of myoglobin enable the early verification of MI.

Objective: Surface plasmon resonance (SPR) based nanosensor was prepared by coating the gold surface of SPR sensor with myoglobin-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)- tryphtophan methyl ester) (PHEMATrp) nanoparticles for the selective and sensitive detection of myoglobin.

Methods: N-methacryloyl-(L)-tryphtophan methyl ester (MATrp) was used as a functional monomer. The myoglobin-imprinted (Myo-MIP) PHEMATrp nanoparticles were prepared by using miniemulsion polymerization. The nanoparticles were characterized by fourier transform infrared spectroscopy, elemental analysis, and Zeta size analysis. Myo-MIP PHEMATrp nanoparticles were attached to the gold surface to prepare the Myo-MIP/SPR nanosensor. The prepared nanosensor was characterized by an atomic force microscope, optic profilometer measurements, a scanning electron microscope, and contact angle measurements. The Myo-MIP/SPR nanosensor’s ability to sense myoglobin was evaluated by using myoglobin solutions (in 20 mM pH 7.4, phosphate buffer) in various concentrations. The data obtained for aqueous myoglobin solution interactions were used to determine the adsorption isotherm and kinetics. Cytochrome c and bovine serum albumin were used to determine the selectivity of the Myo-MIP/SPR nanosensor.

Results: Limit of detection and limit of quantitation values in an aqueous solution were determined to be 4.72 ng / mL and 15.74 ng / mL respectively.

Conclusion: The efficiency of the Myo-MIP/SPR nanosensor was evaluated in a serum sample taken from a patient with acute myocardial infarction and good results were obtained.

Keywords: Molecular imprinting, myocardial infarction, myoglobin, nanosensors, polymeric nanoparticle, SPR.

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Article Details

VOLUME: 2
ISSUE: 2
Year: 2018
Page: [102 - 111]
Pages: 10
DOI: 10.2174/2452271602666180828103017

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