Background: As it is released into the blood during the early stages of myocardial infarction
(MI), myoglobin is one of the best cardiac markers. Its concentration in the blood increases shortly
after MI and reaches a maximum about 12 h after the onset of MI symptoms. These features of myoglobin
enable the early verification of MI.
Objective: Surface plasmon resonance (SPR) based nanosensor was prepared by coating the gold surface
of SPR sensor with myoglobin-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-
tryphtophan methyl ester) (PHEMATrp) nanoparticles for the selective and sensitive detection of myoglobin.
Methods: N-methacryloyl-(L)-tryphtophan methyl ester (MATrp) was used as a functional monomer.
The myoglobin-imprinted (Myo-MIP) PHEMATrp nanoparticles were prepared by using
miniemulsion polymerization. The nanoparticles were characterized by fourier transform infrared
spectroscopy, elemental analysis, and Zeta size analysis. Myo-MIP PHEMATrp nanoparticles were
attached to the gold surface to prepare the Myo-MIP/SPR nanosensor. The prepared nanosensor was
characterized by an atomic force microscope, optic profilometer measurements, a scanning electron
microscope, and contact angle measurements. The Myo-MIP/SPR nanosensor’s ability to sense myoglobin
was evaluated by using myoglobin solutions (in 20 mM pH 7.4, phosphate buffer) in various
concentrations. The data obtained for aqueous myoglobin solution interactions were used to determine
the adsorption isotherm and kinetics. Cytochrome c and bovine serum albumin were used to determine
the selectivity of the Myo-MIP/SPR nanosensor.
Results: Limit of detection and limit of quantitation values in an aqueous solution were determined to
be 4.72 ng / mL and 15.74 ng / mL respectively.
Conclusion: The efficiency of the Myo-MIP/SPR nanosensor was evaluated in a serum sample taken
from a patient with acute myocardial infarction and good results were obtained.