The Antioxidant Potential of Black Cumin (Nigella sativa L.) Extracts Through Different Extraction Methods

Author(s): Muhammad J. Iqbal, Masood S. Butt, Muhammad Sohail, Hafiz A.R. Suleria*.

Journal Name: Current Bioactive Compounds

Volume 15 , Issue 6 , 2019

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background: Herbal foods possess significant amounts of essential bioactive nutrients and offer clinical and therapeutic benefits. The aim of present research was to compare the antioxidant potential of black cumin extracts obtained using different extraction modes.

Materials and Methods: Three solvents (methanol, acetone and hexane) were employed at different time intervals and ratios. However, Supercritical Fluid Extracts (SFE) were obtained at varying pressures (5500, 6500 and 7500 psi).

Results: Outcomes revealed that total phenolics were maximum in methanolic extract (790.79±31.67 mg GAE/100 g) at 50 min. Similarly, DPPH and FRAP activity of methanolic extract was also better than other extracts as 80.99±4.02% and 27.44±0.88 mg TE/g, respectively. HPLC quantification exhibited that highest recovery of major bioactive component (thymoquinone) was obtained via supercritical extraction (12.38 mg/g) at 7500 psi.

Conclusions: It is concluded that Pakistani variety of black cumin possess appreciable antioxidant activity which directly depends upon the extraction conditions. Among conventional solvents, methanol was evidenced to be more efficient. However, supercritical fluid extracts have proved a reliable mean for the extraction of thymoquinone.

Keywords: Black cumin, supercritical, antioxidant potential, HPLC, thymoquinone.

[1]
Bard, M.; Woods, R.A.; Bartón, D.H.; Corrie, J.E.; Widdowson, D.A. Sterol mutants of Saccharomyces cerevisiae: Chromatographic analyses. Lipids, 1977, 12(8), 645-654.
[http://dx.doi.org/10.1007/BF02533759] [PMID: 331007]
[2]
Hussain, S.A.; Hameed, A.; Nazir, Y.; Naz, T.; Wu, Y.; Suleria, H.A.R.; Song, Y. Microencapsulation and the characterization of Polyherbal Formulation (PHF) rich in natural polyphenolic compounds. Nutrients, 2018, 10(7), 843.
[http://dx.doi.org/10.3390/nu10070843] [PMID: 29958444]
[3]
Imran, M.; Ullah, A.; Saeed, F.; Nadeem, M.; Arshad, M.U.; Suleria, H.A.R. Cucurmin, anticancer, and antitumor perspectives: A comprehensive review. Crit. Rev. Food Sci. Nutr., 2018, 58(8), 1271-1293.
[http://dx.doi.org/10.1080/10408398.2016.1252711] [PMID: 27874279]
[4]
Sultan, M.T.; Butt, M.S.; Ahmad, R.S.; Batool, R.; Naz, A.; Suleria, H.A.R. Supplementation of powdered black cumin (Nigella sativa) seeds reduces the risk of hypercholesterolemia. Funct. Food Health Dis., 2011, 1(12), 516-524.
[http://dx.doi.org/10.31989/ffhd.v1i12.109]
[5]
Hameed, I.; Dastagir, G.; Hussain, F. Nutritional and elemental analyses of some selected medicinal plants of the family polygonaceae. Pak. J. Bot., 2008, 40(6), 2493-2502.
[6]
Gharby, S.; Harhar, H.; Guillaume, D.; Roudani, A.; Boulbaroud, S.; Ibrahimi, M.; Ahmad, M.; Sultana, S.; Hadda, T.B.; Chafchaouni-Moussaoui, I.; Charrouf, Z. Chemical investigation of Nigella sativa L. seed oil produced in Morocco. J. Saudi Soc. Agric. Sci., 2015, 14, 172-177.
[http://dx.doi.org/10.1016/j.jssas.2013.12.001]
[7]
Sultan, M.T.; Butt, M.S.; Karim, R.; Ahmad, A.N.; Suleria, H.A.R.; Saddique, M.S. Toxicological and safety evaluation of Nigella sativa lipid and volatile fractions in streptozotocin induced diabetes mellitus. Asian Pac. J. Trop. Dis., 2014, 4, 693-697.
[http://dx.doi.org/10.1016/S2222-1808(14)60709-X]
[8]
Cheikh-Rouhou, S.; Besbes, S.; Lognay, G.; Blecker, C. Deroanne; Attia, H. Sterol composition of black cumin (Nigella sativa L.) and aleppo pine (Pinus halepensis Mill.) seed oils. J. Food Compos. Anal., 2008, 21, 162-168.
[http://dx.doi.org/10.1016/j.jfca.2007.09.001]
[9]
Ramadan, M.F. Nutritional value, functional properties and nutraceuticals applications of black cumin (Nigella sativa L.): An overview. Int. J. Food Sci. Technol., 2007, 42, 1208-1218.
[http://dx.doi.org/10.1111/j.1365-2621.2006.01417.x]
[10]
Gali-Muhtasib, H.; El-Najjar, N.; Schneider-Stock, R. The medicinal potential of black seed (Nigella sativa) and its components. Adv. Phytomed., 2006, 2, 133-153.
[http://dx.doi.org/10.1016/S1572-557X(05)02008-8]
[11]
Khan, S.A.; Khan, A.M.; Karim, S.; Kamal, M.A.; Damanhouri, G.A.; Mirza, Z. Panacea seed “Nigella”: A review focusing on regenerative effects for gastric ailments. Saudi J. Biol. Sci., 2016, 23(4), 542-553.
[http://dx.doi.org/10.1016/j.sjbs.2014.10.001] [PMID: 27298589]
[12]
Dimmitt, S.B.; Stampfer, H.G.; Warren, J.B. Common statin side effects explain poor compliance. Br. J. Clin. Pharmacol., 2015, 80(1), 170-171.
[http://dx.doi.org/10.1111/bcp.12594] [PMID: 25612768]
[13]
Suleria, H.A.R.; Muhammad Jawad, I. Physicochemical and antioxidant properties of pizza dough-base enriched with black cumin (Nigella sativa) extracts. Curr. Nutr. Food Sci., 2018, 14, 1-9.
[14]
Iqbal, M.S.; Ghafoor, A. Inamullah; Abbasi, F.M.; Afsari, S.Q.; Ahmad, H. Study of nutritional characteristics, mineral nutrients and agro-biodiversity in black cumin (Nigella sativa L.) genotypes from Pakistan. Afr. J. Biotechnol., 2011, 10, 14757-14766.
[http://dx.doi.org/10.5897/AJB10.2219]
[15]
Aguilera, J. Food Engineering Interfaces. Food Series Springer Science Business Media LLC, 2011, 393-470.
[http://dx.doi.org/10.1007/978-1-4419-7475-4]
[16]
Ghahramanloo, K.H.; Kamalidehghan, B.; Akbari Javar, H.; Teguh Widodo, R.; Majidzadeh, K.; Noordin, M.I. Comparative analysis of essential oil composition of Iranian and Indian Nigella sativa L. extracted using supercritical fluid extraction and solvent extraction. Drug Des. Devel. Ther., 2017, 11, 2221-2226.
[http://dx.doi.org/10.2147/DDDT.S87251] [PMID: 28814830]
[17]
Herrero, M.; Thornton, P.K.; Notenbaert, A.M.; Wood, S.; Msangi, S.; Freeman, H.A.; Bossio, D.; Dixon, J.; Peters, M.; van de Steeg, J.; Lynam, J.; Parthasarathy Rao, P.; Macmillan, S.; Gerard, B.; McDermott, J.; Seré, C.; Rosegrant, M. Smart investments in sustainable food production: Revisiting mixed crop-livestock systems. Science, 2010, 327(5967), 822-825.
[http://dx.doi.org/10.1126/science.1183725] [PMID: 20150490]
[18]
Mariod, A.A. AL-Naqeb, G.; Ismail, M. Monechma ciliatum methanolic extract regulates low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase genes expression in HepG2 cells. Afr. J. Biotechnol., 2010, 9(36), 5813-5819.
[19]
Solati, A.; Tehrani, A.; Javanbakht, J.; Askari, S.; Hassan, M.A.; Golami, S.; Akbari, H. Haematological studies on broiler chickens fed with different levels of Artemia Urmiana. J. Biotechnol. Biomater., 2012, 2, 4.
[20]
Qiu, Y.; Liu, Q.; Beta, T. Antioxidant properties of commercial wild rice and analysis of soluble and insoluble phenolic acids. Food Chem., 2010, 121, 140-147.
[http://dx.doi.org/10.1016/j.foodchem.2009.12.021]
[21]
Ismail, H.I.; Chan, K.W.; Mariod, A.A.; Ismail, M. Phenolic content and antioxidant activity of cantaloupe (Cucumis melo) methanolic extracts. Food Chem., 2010, 119, 643-647.
[http://dx.doi.org/10.1016/j.foodchem.2009.07.023]
[22]
Iqbal, M.J.; Butt, M.S.; Qayyum, M.M.N.; Suleria, H.A.R. Anti-hypercholesterolemic and anti-hyperglycaemic effects of conventional and supercritical extracts of black cumin (Nigella sativa). Asian Pac. J. Trop. Biomed., 2017, 7(11), 1014-1022.
[http://dx.doi.org/10.1016/j.apjtb.2017.10.005]
[23]
Xu, G.; Ye, X.; Chen, J.; Liu, D. Effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract. J. Agric. Food Chem., 2007, 55(2), 330-335.
[http://dx.doi.org/10.1021/jf062517l] [PMID: 17227062]
[24]
Kim, H.; Moon, J.Y.; Kim, H.; Lee, D.S.; Cho, M.; Choi, H.K. Antioxidant and antiproliferative activities of mango (Mangifera indica L.) flesh and peel. Food Chem., 2010, 121, 429-436.
[http://dx.doi.org/10.1016/j.foodchem.2009.12.060]
[25]
Bourgou, S.; Ksouri, R.; Bellila, A.; Skandrani, I.; Falleh, H.; Marzouk, B. Phenolic composition and biological activities of Tunisian Nigella sativa L. shoots and roots. C. R. Biol., 2008, 331(1), 48-55.
[http://dx.doi.org/10.1016/j.crvi.2007.11.001] [PMID: 18187122]
[26]
Chirinos, R.; Betalleluz, I.; Humána, A.; Arbizub, C.; Pedreschi, R.; Campos, C. HPLC–DAD characterization of phenolic compounds from Andean oca (Oxalis tuberose Mol.) tubers and their contribution to the antioxidant capacity. Food Chem., 2009, 113, 1243-1251.
[http://dx.doi.org/10.1016/j.foodchem.2008.08.015]
[27]
Khattak, K.F.; Simpson, T.J. Ihasnullah, Effect of gamma irradiation on the extraction yield, total phenolic content and free radical-scavenging activity of Nigella staiva seed. Food Chem., 2008, 110(4), 967-972.
[http://dx.doi.org/10.1016/j.foodchem.2008.03.003] [PMID: 26047287]
[28]
Mariod, A.A.; Ibrahim, R.M.; Ismail, M.; Ismail, N. Antioxidant activity and phenolic content of phenolic rich fractions obtained from black cumin (Nigella sativa) seed cake. Food Chem., 2009, 116, 306-312.
[http://dx.doi.org/10.1016/j.foodchem.2009.02.051]
[29]
Sultan, S.; Sheikh-Ali, M.; Alamir, A.; Haas, M.J.; Mooradian, A.D. Uncoupling of hyperglycemia-induced oxidative stress and endoplas mic reticulum stress in endothelial cells. The Endocrine Society Annual Meeting, , Washington, DC . 2009.
[30]
Tubesha, Z.; Iqbal, S.; Ismail, M. Effects of hydrolytic conditions on recovery of antioxidants from methanolic extracts of Nigella sativa seeds. J. Med. Plants Res., 2011, 5, 3152-3158.
[31]
Scherer, R.; Godoy, H.T. Antioxidant Activity Index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chem., 2009, 112, 654-658.
[http://dx.doi.org/10.1016/j.foodchem.2008.06.026]
[32]
Singh, N.; Verma, M.; Mehta, D.; Mehta, B.K. Two new lipid constituents of Nigella sativa (Seeds). Indian J. Chem., 2005, 44, 742-744.
[33]
Abdel-Zaher, A.O.; Abdel-Rahman, M.S.; Elwasei, F.M. Protective effect of Nigella sativa oil against tramadol-induced tolerance and dependence in mice: Role of nitric oxide and oxidative stress. Neurotoxicology, 2011, 32(6), 725-733.
[http://dx.doi.org/10.1016/j.neuro.2011.08.001] [PMID: 21855572]
[34]
Lutterodt, H.; Luther, M.; Slavin, M.; Yin, J.; Parry, J.; Gao, J.; Yu, L. Fatty acid profile, thymoquinone content, oxidative stability, and antioxidant properties of cold-pressed black cumin seed oils. Lebensm. Wiss. Technol., 2010, 43, 1409-1413.
[http://dx.doi.org/10.1016/j.lwt.2010.04.009]
[35]
Martos, G.; López-Fandiño, R.; Molina, E. vitro digestions and IgE binding of proteins from white and whole hen’s egg. Clin. Transl. Allergy, 2011, 1(08)
[36]
Ramadan, M.F.; Asker, M.M.S.; Tadros, M. Antiradical and antimicrobial properties of cold-pressed black cumin and cumin oils. Eur. Food Res. Technol., 2012, 234, 833-844.
[http://dx.doi.org/10.1007/s00217-012-1696-9]
[37]
Szollosi, R.; Varga, I.S. Total antioxidant power in some species of Labiatae (Adaptation of FRAP method). Acta Biol. Szeged., 2002, 46, 125-127.
[38]
Chan, K.W.; Iqbal, S.; Khong, N.M.H.; Babji, A.S. Preparation of deodorized antioxidant rich extracts from 15 selected spices through optimized aqueous extraction. Med. Plants Res., 2011, 5(25), 6067-6075.
[39]
Erkan, N.; Ayranci, G.; Ayranci, E. Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem., 2008, 110(1), 76-82.
[http://dx.doi.org/10.1016/j.foodchem.2008.01.058] [PMID: 26050168]
[40]
el Tahir, K.E.; Ashour, M.M.; al-Harbi, M.M. The cardiovascular actions of the volatile oil of the black seed (Nigella sativa) in rats: Elucidation of the mechanism of action. Gen. Pharmacol., 1993, 24(5), 1123-1131.
[http://dx.doi.org/10.1016/0306-3623(93)90359-6] [PMID: 8270171]
[41]
Kumar, T.V.S.; Negi, P.S.; Sankar, K.U. Antibacterial activity of Nigella sativa L. seed extracts. Br. J. Pharmacol. Toxicol., 2010, 1(2), 96-100.


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 15
ISSUE: 6
Year: 2019
Page: [623 - 630]
Pages: 8
DOI: 10.2174/1573407214666180821124454
Price: $65

Article Metrics

PDF: 19
HTML: 2
EPUB: 1
PRC: 1