Background & Objective: This study investigated the effects of ferulic acid (FR), muscle
exercise (Ex) and combination of them on rotenone (Rot)-induced Parkinson disease (PD) in mice as
well as their underlying mechanisms.
Method: 56 male C57BL/6 mice were allocated into 8 equal groups, 1) Normal control (CTL), 2) FR
(mice received FR at 20 mg/kg/day), 3) Ex (mice received swimming Ex) and 4) Ex + FR (mice received
FR and Ex), 5) Rot (mice received Rot 3 mg/Kg i.p. for 70 days), 6) ROT+ FR (mice received
Rot + FR at 20 mg/kg/day), 7) ROT+ Ex (mice received Rot + swimming Ex) and 8) ROT+ Ex + FR
(mice received Rot + FR and Ex). ROT group showed significant impairment in motor performance
and significant reduction in tyrosine hydroxylase (TH) density and Hsp70 expression (p< 0.05) with
Lewy bodies (alpha synuclein) aggregates in corpus striatum. Also, ROT+FR, ROT+EX and ROT +
Ex+ FR groups showed significant improvement in behavioral and biochemical changes, however the
effect of FR alone was more potent than Ex alone (p< 0.05) and addition of Ex to FR caused no more
significant improvement than FR alone.
Conclusion: We concluded that, FR and Ex improved the motor performance in rotenone-induced PD
rodent model which might be due to increased Hsp70 expression and TH density in corpus striatum
and combination of both did not offer more protection than FR alone.