Background: Tribulus terrestris Linn. (TT) is reported for its ability to improve male
sexual performance, and protodioscin is responsible for the activity. This study was designed to correlate
and compare various extracts of Indian TT with Bulgarian TT, and also the effect of protodioscin
content on the activity.
Methods: Bulgarian TT extract (BT) and prepared solvent extracts [aqueous (WIT), supercritical
fluid (SIT) and methanol (MIT)] of Indian TT were standardized and compared using UV spectrophotometric
method. Forty male rats were randomized into 5 groups of 8 each. Group I served as untreated
control and group II to V were treated with 100 mg/Kg b.wt. of MIT, WIT, BT and SIT, respectively.
The groups received the respective treatment for fourteen days. Sexual behavior of the
rats was observed on Day-1&14. Serum testosterone was estimated after the last observation.
Results: It was found that there was a statistically significant difference between the treatment
groups. However, based on the mean values/ranking analysis of all the parameters, the order of potency
was WIT > SIT ≥ BT > MIT.
Conclusion: These findings indicate that the aqueous extract of Indian TT which contains lesser
protodioscin showed comparatively better sex stimulant activity in comparison to the other tested