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The Natural Products Journal

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ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

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Research Article

Sexual Behaviour in Male Healthy Rats: A Comparison Between Indian Tribulus terrestris Linn. Extracts with Protodioscin Standardized Bulgarian Extract

Author(s): Mohammed Azeemuddin Mukhram, Mohamed Rafiq*, Neeraj Kumar, Atul Namdeorao Jadhav, Suryakanth Dattatray Anturlikar, Ramachandran Sundaram, Uddagiri Venkanna Babu and Rangesh Paramesh

Volume 9, Issue 1, 2019

Page: [32 - 38] Pages: 7

DOI: 10.2174/2210315508666180810144454

Price: $65

Abstract

Background: Tribulus terrestris Linn. (TT) is reported for its ability to improve male sexual performance, and protodioscin is responsible for the activity. This study was designed to correlate and compare various extracts of Indian TT with Bulgarian TT, and also the effect of protodioscin content on the activity.

Methods: Bulgarian TT extract (BT) and prepared solvent extracts [aqueous (WIT), supercritical fluid (SIT) and methanol (MIT)] of Indian TT were standardized and compared using UV spectrophotometric method. Forty male rats were randomized into 5 groups of 8 each. Group I served as untreated control and group II to V were treated with 100 mg/Kg b.wt. of MIT, WIT, BT and SIT, respectively. The groups received the respective treatment for fourteen days. Sexual behavior of the rats was observed on Day-1&14. Serum testosterone was estimated after the last observation.

Results: It was found that there was a statistically significant difference between the treatment groups. However, based on the mean values/ranking analysis of all the parameters, the order of potency was WIT > SIT ≥ BT > MIT.

Conclusion: These findings indicate that the aqueous extract of Indian TT which contains lesser protodioscin showed comparatively better sex stimulant activity in comparison to the other tested extracts.

Keywords: Tribulus terrestris, sexual behaviour, Bulgarian Tribulus, protodioscin, tribulosin, serum testosterone.

Graphical Abstract

[1]
MacKay, D. Nutrients and botanicals for erectile dysfunction: Examining the evidence. Altern. Med. Rev., 2004, 9, 4-16.
[2]
Halvorsen, J.G.; Metz, M.E. Sexual dysfunction, Part I: Classification, etiology, and pathogenesis. J. Am. Board Fam. Pract., 1992, 5(1), 51-61.
[3]
The Ayurvedic Pharmacopeia of India. Ministry of Health and Family Welfare, Govt. of India., 2001, 1, 260.
[4]
Gauthaman, K.; Adaikan, P.G. Effect of Tribulus terrestris on nicotinamide adenine nucleotide phosphate-diaphorase activity and androgen receptors in rat brain. J. Ethnopharmacol., 2005, 96, 127-132.
[5]
Gauthaman, K.; Adaikan, P.G.; Prasad, R.N. Aphrodisiac properties of Tribulus terrestris extract (Protodioscin) in normal and castrated rats. Life Sci., 2002, 71, 1385-1396.
[6]
Gauthaman, K.; Ganesan, A.P. The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction-an evaluation using primates, rabbit and rat. Phytomed. Int. J. Phytother. Phytopharm, 2008, 15, 44-54.
[7]
Do, J.; Choi, S.; Choi, J.; Hyun, J.S. Effects and mechanism of action of a Tribulus terrestris extract on penile erection. Korean J. Urol., 1983, 54, 183-188.
[8]
Protich, M.; Tsvetkov, D.; Nalbanski, B.; Stanislavov, R.; Katsarova, M. Clinical trial of a tribestan preparation in infertile men. Akusherstvo I Ginekol, 2013, 22, 326-329.
[9]
Viktorov, I.; Kaloyanov, A.; Lilov, L.; Zlatanova, L.; Kasabov, V. Clinical investigation on Tribestan in males with disorders in the sexual function; Med-Biol. Inf, 1982.
[10]
Tomowa, M.P.; Botschewa, D.M.; Zaikin, W.G.; Wulfson, N.S. Steroid saponines and sapogenines. V. Hecogenin from Tribulus terrestris L. Planta Med., 1977, 32, 223-224.
[11]
Zheleva-Dimitrova, D.; Nedialkov, P.; Kitanov, G. Radical scavenging and antioxidant activities of methanolic extracts from Hypericum species growing in Bulgaria. Pharmacogn. Mag., 2010, 6, 74-78.
[12]
Adaikan, P.G.; Gauthaman, K.; Prasad, R.N.; Ng, S.C. Pro-erectile pharmacological effects of Tribulus terrestris extract on the rabbit corpus cavernosum. Ann. Acad. Med. Singapore, 2000, 29, 22-26.
[13]
Ganzera, M.; Bedir, E.; Khan, I.A. Determination of steroidal saponins in Tribulus terrestris by reversed-phase high-performance liquid chromatography and evaporative light scattering detection. J. Pharm. Sci., 2001, 90, 1752-1758.
[14]
Dinchev, D.; Janda, B.; Evstatieva, L.; Oleszek, W.; Aslani, M.R.; Kostova, I. Distribution of steroidal saponins in Tribulus terrestris from different geographical regions. Phytochemistry, 2008, 69, 176-186.
[15]
Obreshkova, D.; Pangarova, T.; Mitkov, S.; Dinchev, D. Comparative analytical investigation of Tribulus terrestris preparations. Pharmacia, 1998, 45, 10-12.
[16]
Mahato, S.B.; Sahu, N.P.; Ganguly, A.N.; Miyahara, K.; Kawasaki, T. Steroidal glycosides of Tribulus terrestris Linn. J. Chem. Soc. Perkin Trans, 1981, 1, 2405-2410.
[17]
Yang, D.J.; Lu, T.J.; Hwang, L.S. Simultaneous determination of furostanol and spirostanol glycosides in Taiwanese Yam (Dioscorea spp.) cultivars by high performance liquid chromatography. J Food Drug Anal, 2003, 11, 271-276.
[18]
Singh, S.; Nair, V.; Gupta, Y.K. Evaluation of the aphrodisiac activity of Tribulus terrestris Linn. in sexually sluggish male albino rats. J. Pharmacol. Pharmacother., 2012, 3, 43-47.
[19]
Singh, S.; Gupta, Y.K. Aphrodisiac activity of Tribulus terrestris Linn. in experimental models in rats. J. Men’s Health, 2011, 8, 575-577.
[20]
Yakubu, M.T.; Akanji, M.A. Effect of aqueous extract of Massularia acuminate stem on sexual behaviour of male Wistar rats. Evid. Based Complement. Alternat. Med., 2011, 738103.
[21]
Mills, T.M.; Reilly, C.M.; Lewis, R.W. Androgens and penile erection: A review. J. Androl., 1996, 17, 633-638.
[22]
Thakur, M.; Dixit, V.K. Aphrodisiac activity of Dactylorhiza hatagirea (D. Don) Soo in male albino rats. Evid. Based Complement. Alternat. Med., 2007, 4, 29-31.
[23]
Neychev, V.; Mitev, V. Pro-sexual and androgen enhancing effects of Tribulus terrestris L.: Fact or fiction. J. Ethnopharmacol., 2016, 179, 345-355.
[24]
Augustin, J.M.; Kuzina, V.; Andersen, S.B.; Bak, S. Molecular activities, biosynthesis and evolution of triterpenoid saponins. Phytochemistry, 2011, 72, 435-457.

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