Background and Objectives: Recently S-Allyl-L-Cysteine (SAC) (an organic compound
that is a natural constituent of garlic, is a derivative of the amino acid cysteine) has been introduced as
an agent of suppression of μ- and m-calpain activities and followed by that bright promising neuroprotective
compound. The main objective of this research was to evaluate the biocompatibility and neuroprotective
potential of the encapsulated S-Allyl-L-Cysteine (SAC) into polycaprolactone (PCL)-based
Methods: The copolymer-based SAC-loaded nanocarriers were prepared from coprecipitation method
at constant temperature followed by evaporation of the organic solvent. To the best of our knowledge, it
is the first time to investigate the biocompatibility and neuroprotective potential of SAC loaded into
PEG-b-PCL (poly(ethylene glycol)-block-poly(ε−caprolactone) methyl ether).
Results: The average size of the PEG-b- PCL/empty NCs was 108 nm and for PEG-b-PCL/SAC was
124 nm. The Drug Loading efficiency was 68%. The concentration of PCL-based NCs was 2 x 10 10
particles/ml and the zeta potential of PEG-b- PCL/empty and PEG-b-PCL/SAC NCs was -17 mV and -
23 mV respectively. Biocompatibility and Neuroprotective potential of NCs were evaluated in the SHSY5Y
human neuroblastoma cell line using cell viability and toxicity assays.
Conclusion: The concentration of PEG-b-PCL NCs below 1 x 10 10 particles/ml can be considered as
a safe concentration for the cell line. Also, the SAC encapsulated into PEG-b-PCL NCs has the same
neuroprotective effect as free SAC at lower concentration, and therefore, has a significant neuroprotective
potential against Z-VAD-fmk and St-evoked SH-SY5Y cell damage.