Background: Inflammatory Bowel Diseases (IBD), are considered a growing global disease,
with about ten million people being affected worldwide. Maintenance of intestinal barrier integrity
is crucial for preventing IBD onset and exacerbations. Some recent patents regarding oily
formulations containing probiotics (WO2010122107A1 and WO2010103374A9) and the use of
probiotics for gastrointestinal complaints (US20110110905A1 and US9057112B2) exist, or are
Objective: In this work, we studied the effect of a fixed combination of registered Lactobacillus
reuteri and Lactobacillus acidophilus strains and herbal extracts in an in vitro inflammation experimental
Methods: Caco-2 cell monolayer was exposed to INF-γ+TNF-α or to LPS; Trans Epithelial Electrical
Resistance (TEER) and paracellular permeability were investigated. ZO-1 and occludin Tight
Junctions (TJs) were also investigated by mean of immunofluorescence.
Results: Pre-treatment with the fixed combination of probiotics and herbal extracts prevented the
inflammation-induced TEER decrease, paracellular permeability increase and TJs translocation.
Conclusions: In summary, the fixed combination of probiotics and herbal extracts investigated in
this research was found to be an interesting candidate for targeting the re-establishment of intestinal
barrier function in IBD conditions.