Background: Tuberculosis is a greatest threat to human health. It requires urgent
need to seek new devise alternate strategies and ant-tubercular compounds. In the
present scenario, Nonmaterias, have opened new avenues in medicine, diagnosis and
Objective: In view of this, the current study aims to synthesize gold nanoparticles and determine
its efficacy to inhibit Mycobacterium tuberculosis.
Method: Gold nanoparticles (GNPs) synthesized from medicinal plant, such as Ocimum
gratissimum Linn, were tested against Mycobacterium tuberculosis (H37RV strain). Gold
nanoparticles were characterized by UV-Vis spectrophotometer, FTIR, SEM and TEM.
TEM results revealed that the GNPs were found spherical in structure and around 10-25
nm in diameter. UV-Vis spectroscopy exhibited an absorption peak at 348 nm. Fourier
transform infra-red spectroscopy showed the GNPs have coated with phytoconstituents
(terpenoids) that indicate the role of bio-molecules responsible for efficient stabilization
and capping of the gold nanoparticles. In vitro model was designed to determine minimum
inhibitory concentration (MIC) of each sample by Lowenstein Jensen (LJ) slope method.
Results: The results showed that the presence of ursolic acid in ethanolic and hydroalcoholic
extracts was found to be 2.89 % and 1.97 %, respectively. GNPs of ethanolic and
hydroalcoholic exhibited anti-tubercular activity, with MIC 2.5 μg/ml and 20 μg/ml, respectively.
While ethanolic and hydroalcoholic extracts showed such activity at concentrations
50 μg/ml and 75 μg/ml, respectively.
Conclusion: GNPs synthesized from ethanolic extract showed profound efficiency to kill
mycobacteria. As in this method no chemical reagents were used, the synthesized gold
nanoparticles have potential for biological applications. There is an urgent need to further
development of nano-antibiotic for tuberculosis.