Adjuvant Activity of Bursal Pentapeptide-(III-V) in Mice Immunized with the H9N2 Avian Influenza Vaccine

Author(s): Jiangfei Zhou, Cong Zhang, Zhixin Liu, Yongqing Liu, Kairui Cai, Tengfei Shen, Chengshui Liao*, Chen Wang*.

Journal Name: Protein & Peptide Letters

Volume 25 , Issue 8 , 2018

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Abstract:

Background: Bursal Pentapeptide (BPP)-(III-V) are peptides isolated from the Bursa of Fabricius (BF).

Method: The peptides' adjuvant activities were evaluated in mice immunized with the H9N2 avian influenza vaccine.

Results: The results showed that BPP-IV did not promote humoral or cellular immune responses. BPP-III had no effect on antibody production, but increased populations of CD3+ and CD8+ T cells, CTL levels and proliferation activities of splenic T-lymphocytes. BPP-V increased the level of antibodies (HI, IgG and its subtype IgG1) and the cytokine IL-4, as well as the ratio of CD3+ T cells and its subsets CD4+ and CD8+ T cells, BPP-V also increased CTL level and promoted the proliferation activity of T- and B-lymphocyte. Furthermore, in animal challenge experiment, BPP-III and BPP-V reduced viral RNA copies and viral titers, and reduced inflammatory cell infiltration.

Conclusion: In summary, BPP-IV did not have an effect on specific immune responses, therefore it could not reduce pathological damage in mice lungs; BPP-III provided resistance to the antigen through the cell-mediated immune response; BPP-V enhanced both humoral and cellular immune responses, therefore provided protections against the H9N2 Avian Influenza Virus (AIV). Altogether, BPP-III and BPP-V could be adjuvant candidates for the H9N2 avian influenza vaccine.

Keywords: Bursal pentapeptided-(III-V), adjuvants, humoral immune response, cellular immune response, immunoprotection test, avian influenza virus.

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Article Details

VOLUME: 25
ISSUE: 8
Year: 2018
Page: [757 - 766]
Pages: 10
DOI: 10.2174/0929866525666180806110928
Price: $58

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