Frontiers in Drug Safety

Frontiers in Drug Safety

Adulteration Analysis of Some Foods and Drugs

Adulteration refers to the practice of altering food or pharmaceutical content to reduce production costs. Factors affecting this practice include market forces such as easy availability of food ...
[view complete introduction]

US $

*(Excluding Mailing and Handling)

Inspection of Colorant Adulteration by Modern LC Mass Spectrometry

Pp. 37-64 (28)

Mingchih Fang, Chia-Fen Tsai and Hwei-Fang Cheng


This chapter introduces modern mass spectrometry for the detection of dyes in foods. An LC/MS/MS method for the determination of 20 synthetic dyes is first described. Followed high resolution mass spectrometry (HRMS) demonstrates the modern screening method for illegal dye adulteration. There is an example of semitargeted/ non-targeted detection and structure confirmation of a non-permitted dye, diethyl yellow, by quadrupole-orbitrap HRMS. Mass spectrometry operated on selected reaction monitoring (SRM) is gold standard normally used for the detection of analytes such as pesticides, veterinary medicines and additives such as dyes in complex matrix. However, SRM only allows signals which fit target list to be recorded. It will lose all information of semi-target, non-target or even unknown. A new concept called dataindependent acquisition (DIA) overcomes the limitations of SRM and increases the possibility of semi-target and non-target detection. This chapter includes the application of DIA for simultaneously screening and confirmation of dyes in various foods.


Adulteration, Carminic acid, Colorants, Data-independent acquisition, Dye, Non-targeted, Orbitrap, Semi-targeted, Sudan dyes, Sulforhodamine B.


Division of Research and Analysis, Taiwan Food and Drug Administration, Taipei City, Taiwan.