Background: Small double-strand RNAs (dsRNAs) molecules are able to activate endogenous
genes via an RNA-based promoter-targeting mechanism. Like RNA interference (RNAi), RNA
activation (RNAa) is an evolutionarily conserved mechanism that is present in diverse eukaryotic organisms
ranging from yeast to humans.
Methods: The small activating RNAs (saRNAs) that are involved in RNAa have been successively
used to activate gene expression in cultured cells. Thus, this emergent technique might allow us to develop
biotechnological and therapeutic applications without the need to synthesize hazardous construct
systems that harbor exogenous DNA sequences.
Result and Conclusion: Accordingly, this article aims to provide insights into how RNAa cellular machinery
can be manipulated to activate gene expression and for more effective clinical treatments of