Spatio-Temporal Expression and Functional Analysis of miR-206 in Developing Orofacial Tissue

Author(s): Partha Mukhopadhyay, Irina Smolenkova, Dennis Warner, Michele M. Pisano, Robert M. Greene*.

Journal Name: MicroRNA

Volume 8 , Issue 1 , 2019

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Abstract:

Background: Development of the mammalian palate is dependent on precise, spatiotemporal expression of a panoply of genes. MicroRNAs (miRNAs), the largest family of noncoding RNAs, function as crucial modulators of cell and tissue differentiation, regulating expression of key downstream genes.

Observations: Our laboratory has previously identified several developmentally regulated miRNAs, including miR-206, during critical stages of palatal morphogenesis. The current study reports spatiotemporal distribution of miR-206 during development of the murine secondary palate (gestational days 12.5-14.5).

Result and Conclusion: Potential cellular functions and downstream gene targets of miR-206 were investigated using functional assays and expression profiling, respectively. Functional analyses highlighted potential roles of miR-206 in governing TGFß- and Wnt signaling in mesenchymal cells of the developing secondary palate. In addition, altered expression of miR-206 within developing palatal tissue of TGFß3-/- fetuses reinforced the premise that crosstalk between this miRNA and TGFß3 is crucial for secondary palate development.

Keywords: Embryo, fetus, microRNA, miR-206, mouse, palate.

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VOLUME: 8
ISSUE: 1
Year: 2019
Page: [43 - 60]
Pages: 18
DOI: 10.2174/2211536607666180801094528
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