Background: It is well documented in the scientific literature that high blood pressure can
lead to cardiovascular disease. Untreated hypertension has clinical consequences such as coronary artery
disease, stroke or kidney failure. Diltiazem hydrochloride (DH), a calcium-channel blocker, and
perindopril erbumine (PE), an inhibitor of the angiotensin converting enzyme are used for the management
Objective: This project will examine the effect of microneedle rollers on the transport of DH and PE
across pig ear skin. The use of the transcutaneous route of administration reduces and in sometimes
eliminates the trauma and pain associated with injections. Furthermore, there is increased patient compliance.
The purpose of this project was to study the effect of stainless steel microneedles on the transdermal
delivery of DH and PE.
Method: We utilized vertical Franz diffusion cells to study in vitro transport of DH and PE across microneedle-
treated pig ear skin. Confocal laser scanning microscopy (CLSM) was used to characterize
microchannel depth. Transdermal flux values were determined from the slope of the linear portion of
the cumulative amount versus time curve.
Results: There was a 113.59-fold increase in the transdermal permeation of DH following the application
of microneedle roller compared to passive diffusion.
Conclusion: In the case of PE, there was an 11.99-fold increase in the drug transport across pig skin
following the application of microneedle rollers in comparison with passive diffusion. Student’s t-test
and Mann-Whitney’s rank sum test were used to determine statistically significant differences between
experimental and control groups.