Objective: The aim of this study was to evaluate the influence of adalimumab on the
expression pattern of genes associated with JAK/STAT signaling pathway in Normal Human Dermal
Fibroblast (NHDF) cells stimulated with 8 µg/ml of adalimumab and the identification of miRNAs
regulating these genes’ expression.
Method: NHDFs were cultured with or without the presence of adalimumab for 2, 8, and 24h. The microarray
technology was used to determine expression profile of mRNAs and miRNAs.
Results: Out of 22283 ID mRNA, 37 are associated with the JAK/STAT signaling pathway. It can be
observed that 18 mRNAs differentiate NHDFs cultures with adalimumab from control. The analysis of
miRNAs showed that, among 1105 ID miRNAs, 20 miRNAs are differentiating in cells treated with
adalimumab for 2h, 9 miRNAs after 8h, and only 3 miRNAs after 24h.
Conclusion: It can be observed that miRNAs play an extremely important role in the regulation of the
expression of genes associated with JAK/STAT signaling pathway. The results of this study show the
possibility of using changes in mRNAs and miRNAs profile expression, as complementary molecular
markers of adalimumab treatment effectiveness.