Differentially Expressed Wound-response-related Proteins from a Major Agarwood-producing Tree, Aquilaria malaccensis Lam. Identified via 2-D Electrophoresis

Author(s): Shiou Yih Lee , Samsuddin Ahmad Syazwan , Dhilia Udie Lamasudin , Rozi Mohamed* .

Journal Name: Current Proteomics

Volume 15 , Issue 4 , 2018

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Abstract:

Background: Wounding is the major trigger factor for agarwood formation in Aquilaria malaccensis. To date, the mechanism of agarwood formation in Aquilaria has been extensively explored through anatomy and genomics approaches; however, at the protein level, it is yet to be reported.

Objective: A comparative proteomics analysis was carried out to identify proteins responsive to wounding in A. malaccensis, using two-dimensional gel electrophoresis (2-DE) coupled with Matrix- Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).

Method: Proteins were extracted from A. malaccensis tree stems at 0, 6, 12, and 24 h after wounding and separated by 2-DE, sequenced using MALDI-TOF MS and identified through UniProt/Swiss-Prot protein database.

Results: Changes in differentially displayed proteins were observed. A total of 15 protein spots were identified reproducible under 2-DE, of which only two protein spots showed differences in expression affected by the wounding treatment in a time-dependent experiment. They were predicted as Malate Synthase (MS) and the reduced Nicotinamide Adenine Dinucleotide Phosphate (NADPH) quinone oxidoreductase subunit 2 B. Both proteins were directly and indirectly related to wounding treatments in plants and may be related to agarwood formation mechanism.

Conclusion: As Aquilaria proteome is not available to date, this first report on differential protein expression in response to wounding could serve as a useful information towards understanding agarwood biosynthesis at the protein level.

Keywords: Agarwood, gaharu, 2-DE, MALDI-TOF, proteome, electrophoresis, wound.

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Article Details

VOLUME: 15
ISSUE: 4
Year: 2018
Page: [291 - 298]
Pages: 8
DOI: 10.2174/1570164615666180727095937
Price: $58

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