Background: Wounding is the major trigger factor for agarwood formation in Aquilaria
malaccensis. To date, the mechanism of agarwood formation in Aquilaria has been extensively explored
through anatomy and genomics approaches; however, at the protein level, it is yet to be reported.
Objective: A comparative proteomics analysis was carried out to identify proteins responsive to
wounding in A. malaccensis, using two-dimensional gel electrophoresis (2-DE) coupled with Matrix-
Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).
Method: Proteins were extracted from A. malaccensis tree stems at 0, 6, 12, and 24 h after wounding
and separated by 2-DE, sequenced using MALDI-TOF MS and identified through UniProt/Swiss-Prot
Results: Changes in differentially displayed proteins were observed. A total of 15 protein spots were
identified reproducible under 2-DE, of which only two protein spots showed differences in expression
affected by the wounding treatment in a time-dependent experiment. They were predicted as Malate
Synthase (MS) and the reduced Nicotinamide Adenine Dinucleotide Phosphate (NADPH) quinone oxidoreductase
subunit 2 B. Both proteins were directly and indirectly related to wounding treatments in
plants and may be related to agarwood formation mechanism.
Conclusion: As Aquilaria proteome is not available to date, this first report on differential protein expression
in response to wounding could serve as a useful information towards understanding agarwood
biosynthesis at the protein level.