Background: Although Pichia pastoris is an outstanding host among conventional expression
systems for production of recombinant proteins, a new interest has been emerged to this system
due to the inherent advantages and new developments in this expression host. The potential for secretory
and soluble expression of heterologous glycoproteins in P. pastoris proposed this system as a candidate
for the production of complex eukaryotic proteins.
Methods: Several new developments have occurred in different areas related to P. pastoris expression
system including hosts, vectors, glycosylation pattern and fermentation technology. Strain engineering
using Crispr/Cas9 technology to produce human-like glycoproteins and protease deficient strains are
two new areas of development with high importance.
Results: This review is dedicated to discuss the most important characteristics of P. pastoris with emphasis
on new developments, especially in the field of glycoengineering, efficient expression vectors
Conclusion: New developments that occurred in the P. pastoris expression system converted this system
to a versatile host for the production of complex proteins. This progress paved the way for several
proteins to enter the clinical trials or industrial processes with this valuable expression host.