LncRNA MALAT1 is Neuroprotective in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury Through miR-204 Regulation

Author(s): Yong Qiao, Changliang Peng, Ji Li, Dongjin Wu, Xiuwen Wang*.

Journal Name: Current Neurovascular Research

Volume 15 , Issue 3 , 2018

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Abstract:

Background: This study was to investigate the neuroprotective effect of long noncoding RNAs Metastasis associated lung adenocarcinoma transcript-1 (lncRNA MALAT-1) in Spinal Cord Ischemic/Reperfusion Injury (SCIRI).

Methods: Quantitative real-time PCR (RT-qPCR) was used to examine the expressions of MALAT1, miR-204 and Bcl-2, while western blot was performed to examine Bcl-2. Besides, apoptosis was evaluated by the percentage of cell viability and apoptotic cells. Neurological evaluation was performed by measuring hindlimb locomotor function.

Results: The expression of MALAT1 and Bcl-2 was decreased, while miRNA-204 (miR-204) was up-regulated in rats SCIRI model and neurocyte lines under hypoxic conditions. Oxygen, Glucose Deprivation (OGD) promoted apoptosis of neurocytes, downregulated MALAT1 and Bcl-2 and elevated miR-204 expression, however, overexpression of MALAT1 notably reversed this trend. Nevertheless, knockdown of MALAT1 increased cell apoptosis, decreased MALAT1 and Bcl-2 but upregulated miR-204. MALAT1 overexpression-induced anti-apoptosis and knockdowninduced pro-apoptosis were obviously reversed by synchronously overexpression and knockdown of miR-204, respectively. MALAT1-treated SCIRI rats exhibited lower Motor Deficit Index (MDI) scores, higher levels of MALAT1 and Bcl-2 expression and lower miR-204 expression.

Conclusion: Our data suggested that MALAT1 exerted neuroprotective effect in a rat model of SCIRI by regulating miR-204.

Keywords: MALAT-1, miR-204, spinal cord, ischemia/reperfusion injury, oxygen, glucose deprivation.

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Article Details

VOLUME: 15
ISSUE: 3
Year: 2018
Page: [211 - 219]
Pages: 9
DOI: 10.2174/1567202615666180712153150
Price: $58

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