Notoginsenoside R1 Promotes the Growth of Neonatal Rat Cortical Neurons via the Wnt/β-catenin Signaling Pathway

Author(s): Xing-Tong Li, Wei Ma, Xian-Bin Wang, Zhang Liang, Jin-Wei Yang, Yuan He, Yun-Fei Dai, Tong Zhang, Jia-Wei Wang, Kuang-Pin Liu, Xiang-Peng Wang, Xing-Kui Zhang, Chun-Yan Li, Jun-Jun Li, Hong-Yan Tang, Ping Su*, Jian-Hui Guo*, Li-Yan Li*.

Journal Name: CNS & Neurological Disorders - Drug Targets

Volume 17 , Issue 7 , 2018

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Abstract:

Background & Objective: Notoginsenoside R1 (NGR1) is one of the main effective components of Panax notoginseng.

Method: Primary cortical neurons were harvested from neonatal rats and cultured to analyze the role of NGR1 in neuronal growth and the effects of NGR1 on the Wnt/β-catenin signaling pathway. Following treatment with NGR1, immunocytochemistry was used to detect expression of Tuj1 and MAP2, and RT-qPCR was used to measure mRNA levels of key factors in the Wnt signaling pathway.

Results: Results showed that NGR1 promotes growth of cultured neurons and significantly upregulates mRNA levels of β-catenin, Dishevelled, and Frizzled. To further confirm whether NGR1 promoted cortical neuron growth via the Wnt/β-catenin signaling pathway, we knocked down β- catenin mRNA by siRNA interference; following NGR1 treatment of β-catenin-knockdown neurons, β-catenin mRNA levels increased significantly.

Conclusion: In conclusion, these results demonstrate that NGR1 promotes growth of cultured cortical neurons from the neonatal rat, possibly via the Wnt/β-catenin signaling pathway.

Keywords: Wnt/β-catenin signaling pathway, β-catenin, NGR1, growth, cortical neuron, RNA interference.

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Article Details

VOLUME: 17
ISSUE: 7
Year: 2018
Page: [547 - 556]
Pages: 10
DOI: 10.2174/1871527317666180711093538
Price: $58

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