Impact of Electron Beam Irradiation on the Bioactive Principles of Seeds of Coastal Sand Dune Wild Legumes (Canavalia spp.)

Author(s): Prabhavathi Supriya, Kandikere R. Sridhar*.

Journal Name: Recent Patents on Food, Nutrition & Agriculture

Volume 10 , Issue 1 , 2019

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Graphical Abstract:


Background: Recent patents reveal that vegetable ingredients have several applications in novel food formulations. Many so-called antinutritional components (e.g. tannins, saponins, lectins and protease inhibitors) have nutraceutical as well as pharmaceutical significance. Seeds of two wild legumes of the genus Canavalia inhabitants of the coastal sand dunes of Southwest India are known for a variety of bioactive principles (e.g. phenolics, tannins, canavanine, concanavalin and phytohemagglutinins).

Objective: This study evaluates the impact of Electron Beam (EB) irradiation on the bioactive components of seeds of two coastal sand dune wild legumes Canavalia cathartica and C. maritima.

Methods: The dry seeds of C. cathartica and C. maritima were EB irradiated with different doses (2.5, 5, 10 and 15 kGy) to follow changes in six bioactive principles (total phenolics, orthodihydric phenols, tannins, canavanine, trypsin inhibitors and phytohemagglutinins) in comparison to control seeds. One-way ANOVA was employed to follow the variation in bioactive components of seeds in control and different doses of irradiation.

Results: Seeds of both legumes were devoid of orthodihydric phenols and trypsin inhibitors. In C. cathartica, the total phenolics showed significant dose-dependent increase up to 5 kGy and decreased thereafter. Tannin content was not altered up to 10 kGy followed by significant decrease at 15 kGy. There was no significant change in canavanine content and the phytohemagglutinin activity against human erythrocytes was not altered. Seeds of C. maritima did not show significant changes in total phenolics as well as tannin contents. The content of canavanine showed significant dose-dependent increase up to 5 kGy followed by significant decrease. There was no variation in phytohemagglutinin activity against erythrocytes A, B and O, while against AB, the activity decreased at 2.5 kGy and further decrease was constant at higher doses.

Conclusion: The EB irradiation doses employed have selectively altered the bioactive principles of Canavalia seeds and such treatments may facilitate to maneuver desired medicinal, nutritional, functional and cooking properties. Besides selective changes in bioactive components the seeds have extended shelf life.

Keywords: Canavanine, hemagglutinin, phenolics, seeds, trypsin inhibition, wild legumes.

Vadivel V, Janardhanan K. Chemical composition of the underutilized legume Cassia hirsuta L. Plant Foods Hum Nutr 2000; 55: 369-81.
Siddhuraju P, Becker K. Species/variety differences in biochemical composition and nutritional value of Indian tribal legumes of the genus Canavalia. Nahrung 2001; 45: 224-33.
Thangadurai D, Murthy KSR, Pullaiah T. Characterization, Conservation and Utilization of Plant Genetic Resources for Future Food, Agriculture and Medicine. In: Trivedi PC, Ed;Biodiversity Assessment and Conservation. Springer Verlag: Berlin 2006; pp. 247-63.
Thangadurai D, Viswanathan MB, Ramesh N. The chemical composition and nutritional evaluation of Canavalia virosa: A wild perennial bean from Eastern Ghats of Peninsular India. Eur Food Res Technol 2001; 213: 456-9.
Siddhuraju P, Makkar HPS, Becker K. The effect of ionizing radiation on antinutritional factors and the nutritional value of plant materials with reference to human and animal food. Food Chem 2002; 78: 187-205.
Murthy KSR, Rani SS, Pullaiah T. Wild edible plants of Andhra Pradesh, India. J Econ Taxon Bot 2003; 27: 613-30.
Vijayakumari K, Pugalenthi M, Vadivel V. Effects of soaking and hydrothermal processing methods on the levels of antinutrients and in vitro protein digestibility of Bauhinia purpurea L. seeds. Food Chem 2007; 103: 968-75.
Bhat R, Sridhar KR, Seena S. Nutritional quality evaluation of velvet bean seeds (Mucuna pruriens) exposed to gamma irradiation. Int J Food Sci Nutr 2008; 59: 261-78.
Sridhar KR, Shreelalitha SJ, Supriya P, Arun AB. Nutraceutical attributes of ripened split beans of three Canavalia landraces. Agric Technol 2016; 7: 1277-97.
Arun AB, Raviraja NS, Sridhar KR. Effect of temperature, salinity and burial on seed germination and seedling emergence of five coastal sand dune legumes. Int J Ecol Environ Sci 2001; 27: 23-9.
Seena S, Sridhar KR. Nutritional and microbiological features of little known legumes, Canavalia cathartica Thouars and C. maritima Thouars of the southwest coast of India. Curr Sci 2006; 90: 1638-50.
Sridhar KR, Seena S. Nutritional and antinutritional significance of four unconventional legumes of the genus Canavalia - a comparative study. Food Chem 2006; 99: 267-88.
Supriya P, Sridhar KR, Nareshkumar S, Ganesh S. Impact of electron beam irradiation on fatty acid profile of Canavalia seeds. Food Bioprocess Technol 2012; 5: 1049-60.
D’Cunha M, Sridhar KR. Agrobotanical traits of wild legumes Canavalia on the coastal sand dunes. Agric Technol 2013; 9: 1821-36.
D’Cunha M, Sridhar KR, Bhat R. Nutritional quality of germinated seeds of Canavalia maritima of coastal sand dunes.Food Processing: Methods, Techniques and TrendsBellinghouse VC, Ed;. Nova Science Publishers Inc: New York 2009; pp. pp. 363-384.
D’Cunha M, Sridhar KR. L-canavanine and L-arginine in two wild legumes of the genus Canavalia. Ins Intrgr Omics Appl Biotechnol J 2010; 1: 29-33.
Fricke H, Hart EJ. Chemical dosimetry.In: Attix FH, Raesch WC,Ed; Radiation Dosimetry. Academic Press: New York 1966; pp. pp. 167-239.
Gupta BL, Narayan GR, Nilekani SR, Bhat RM, Kaul A, Bemalkhedkar MM. Preliminary dosimetry studies for a Microtron using chemical dosimeters. J Rad Prot Environ 1999; 22: 169-74.
Rosset J, Bärlocher F, Oertli JJ. Decomposition of conifer needles and deciduous leaves in two Black Forest and two Swiss Jura streams. Int Rev Gesamten Hydrobiol 1982; 67: 695-711.
Mahadevan A, Sridhar R. Methods in Physiological Plant Pathology. 3rd ed. Sivakami Publications: Chennai, India 1985.
Burns R. Methods for estimation of tannins in grain sorghum. Agron J 1971; 63: 511-2.
Fearon WR, Bell EA. Canavanine: Detection and occurrence in Colutea arborescens. J Biochem 1954; 59: 221-4.
Kakade ML, Rackis JJ, McGhee JE, Puski G. Determination of trypsin inhibitor activity of soy products, a collaborative analysis of an improved procedure. Cereal Chem 1974; 51: 376-82.
Occenã IV, Majica E-RE, Merca FE. Isolation of partial characterization of a lectin from the seeds of Artocarpus camansi Blanco. Asian J Plant Sci 2007; 6: 757-64.
Liener IE. Implications of antinutritional components in soybean foods. Crit Rev Food Sci Nutr 1994; 34: 31-67.
Thomas FA, Rosenthal GA, Gold DV, Dickey K. Growth inhibition of a rat colon tumor by L-canavanine. Canc Res 1986; pp. pp. 2898-.
Enneking D, Wink M. Towards the elimination of anti-nutritional factors in grain legumes.In: Current Plant Science and Biotechnology in Agriculture, Volume # 34Knight R, Ed;. Kluwer Academic Publishers: Dordrecht 2000; pp. pp. 375-384.
Combs GF, Gray WP. Chemopreventive agents: Selenium. Pharmacol Ther 1998; 79: 179-92.
Bhat R, Sridhar KR. Shelflife improvement and value addition of nutraceutically valued legume - Mucuna through application of ionizing radiation. International Conference on Integration of Science and Technology for Sustainable Development Faculty of AgriculturalTechnology, King Mongkut’s Institute of TechnologyLadkrabang, Bangkok. 1-16.
Bhat R, Sridhar KR. Influence of electron beam irradiation on the antinutritional features and protein digestibility of seeds of nutraceutically valued legume Mucuna pruriens. In food processing: Methods, techniques and trends; Bellinghouse VC, Ed; Nova Science Publishers Inc 2009; New York 2009. 197-209.
Thurn MJ, Huang LJ. Methods for treating cancer with legume plant extracts. US09030704 1999.
Bhat R, Sridhar KR, Young C-C, Arun AB, Ganesh S. Composition and functional properties of raw and electron beam irradiated Mucuna pruriens seeds. Int J Food Sci Technol 2008; 43: 1338-51.
Supriya P, Sridhar KR, Ganesh S. Fungal decontamination and enhancement of shelf life of edible split beans of wild legume Canavalia maritima by the electron beam irradiation. Radiat Phys Chem 2014; 96: 5-11.

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Article Details

Year: 2019
Page: [57 - 61]
Pages: 5
DOI: 10.2174/2212798410666180709144200
Price: $58

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