Background: Radiation therapy is widely used for the treatment of pituitary adenomas.
Unfortunately, it might raise the risk of ischemic stroke, with neuroinflammation being a major pathological
process. Astrocytes are the most abundant cell type in the central nervous system and have
been reported for playing important roles in ischemic stroke.
Objective: Here we studied how γ-radiation would introduce astrocytes into a detrimental state for
neuroinflammation and provide new theory evidence and target for the clinical management of inflammation-
related neural damage after radiation-induced ischemic stroke.
Method: HA-1800 cells were treated with γ-radiation and then the protein and mRNA levels of Connexin
(Cx)-43 were evaluated by western and q-PCR. The culture supernatant was collected and the
concentrations of the inflammatory factors were determined by ELISA. MiRNA complementary to
Cx-43 was designed through the online tools.
Results: Cx-43 is upregulated in the treatment of γ-radiation in astrocytes and γ-radiation introduced
the detrimental function of astrocytes: cell viability was reduced while the apoptotic cells were increased.
Inflammatory factors like tumor necrosis factor alpha, interferon gamma, interleukin-6, interleukin
1-beta were dramatically up-regulated by the irradiation. MiR-374a rescued irradiation induced
Cx-43 up-regulation of astrocytes and eliminated detrimental function triggered by γ-radiation.
Conclusion: Cx-43 expression level may play an important role in the inflammation-related neural
damage after irradiation-induced ischemic stroke.