Background: Previously, we showed that the Zinc finger-containing
transcription factor ZNF424 inhibits p21 transcription, which has been widely associated
with various cancers. However, because the roles of ZNF424 in tumorigenesis have not
been characterized, we correlated ZNF424 expression with tumorigenesis in lung
Results: The present immunohistochemical analyses show significantly lower ZNF424
expression levels in 43 of 60 lung cancer tissues compared with adjacent tissues.
Moreover, flow cytometry assays indicated that overexpression of ZNF424 induces
apoptosis in A549 human lung carcinoma cells, and overexpression of ZNF424
significantly increases numbers of G1 phase cells and decreases numbers of S phase
cells, suggesting that ZNF424 inhibits proliferation. Western Blot analyses show that
overexpression of ZNF424 decreases protein expression levels of the mitogen-activated
protein kinase (MAPK) signaling proteins P-P38 and P-ERK in A549 cells.
Conclusion: These are the first data to associate ZNF424 with tumorigenesis and
demonstrate an inhibitory role in lung cancer, indicating the potential of ZNF424
expression as a diagnostic marker of lung tumorigenesis.