Protein-Nucleic Acid Interactions
Pp. 76-84 (9)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
The biological network is intricately woven by several entities which work
in cellular system in a co-ordinated manner as a result of different types of interactions
(protein-protein interactions, protein-RNA and protein-DNA interactions). Among
them, the protein-nucleic interactions play an important role in regulating cell function
and disruption among these interactions leads to catastrophic consequences within the
biological system. The protein-nucleic acid interactions are integrated into several key
cellular processes which include regulation of gene expression, replication,
recombination, repair, translation, transcription, packaging of nucleic acids and the
formation of cellular machinery. In this chapter, we will discuss the several methods by
which we can analyse protein-nucleic acid interactions.
Chromatin Immunoprecipitation, EMSA, Gel Shift Assays, miRNAs,
SDS-PAGE, Transcriptional factors, Untranslated regions (UTR).
Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.