Evaluation of Cellular Processes by In Vitro Assays

Evaluation of Cellular Processes by In Vitro Assays

This handbook presents information on different cell culture assays which can be used to perform experimental analysis. Readers are introduced to the basics of in vitro cell cultures followed by a ...
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Protein-Protein Interactions

Pp. 68-75 (8)

Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq

Abstract

Protein-protein interactions are central to every cellular process like DNA replication, transcription, translation, splicing, signal transduction and cell cycle control. To elucidate the function of a gene, we need to determine the function of the gene’s encoded protein product. Identifying the role of a protein needs understanding about the proteins that interact with each other for functioning of a particular biological pathway. Assessment about function of a protein can be made by understanding protein-protein interaction studies. These inferences are based on the fact that the role of unknown proteins may be elucidated if captured through their interaction with a protein target of known function. Thus in this chapter, we attempt to summarize different in vitro methods used to identify proteins- protein interactions and to assess the strengths of these interactions.

Keywords:

Agarose Beads, Co-Immunoprecipitation, Far Western Blotting, Monoclonal Antibody, Protein A, Pull Down Assay, SDS-PAGE, Sepharose.

Affiliation:

Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.