Pp. 68-75 (8)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
Protein-protein interactions are central to every cellular process like DNA
replication, transcription, translation, splicing, signal transduction and cell cycle
control. To elucidate the function of a gene, we need to determine the function of the
gene’s encoded protein product. Identifying the role of a protein needs understanding
about the proteins that interact with each other for functioning of a particular biological
pathway. Assessment about function of a protein can be made by understanding
protein-protein interaction studies. These inferences are based on the fact that the role
of unknown proteins may be elucidated if captured through their interaction with a
protein target of known function. Thus in this chapter, we attempt to summarize
different in vitro methods used to identify proteins- protein interactions and to assess
the strengths of these interactions.
Agarose Beads, Co-Immunoprecipitation, Far Western Blotting,
Monoclonal Antibody, Protein A, Pull Down Assay, SDS-PAGE, Sepharose.
Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar, India.